{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:46:39Z","timestamp":1791895599076,"version":"4.3.4"},"reference-count":9,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,4,6]],"date-time":"2018-04-06T00:00:00Z","timestamp":1522972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2016A030313471"],"award-info":[{"award-number":["2016A030313471"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009330","name":"Medical Science and Technology Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["A2017311"],"award-info":[{"award-number":["A2017311"]}],"id":[{"id":"10.13039\/501100009330","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acne is a common chronic skin disease with a multifactorial aetiology and pathogenesis. Recently, circular <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) have been identified as a key factor in regulating gene expression through circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2013mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> networks in many biological processes and human diseases. However, the circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s expression in patients with acne is still unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression profile in severe acne.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression profile of circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in three paired lesional skin and adjacent non\u2010lesional skin in severe acne was detected by high\u2010throughput <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> sequencing technology and bioinformatics analysis. The candidate circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were validated by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, Sanger sequencing and <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in the separate group (<jats:italic>n<\/jats:italic> = 4). The circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2013mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> interaction networks were predicted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 538 circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s including 271 up\u2010 and 267 downregulated circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were differentially expressed in lesional skin compared with adjacent non\u2010lesional skin in severe acne. Gene Ontology and <jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content> pathway enrichment analyses revealed that the aberrantly expressed circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were primarily involved in inflammatory, metabolism and immune responses. Five candidate circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>_0084927, circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>_0001073, circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>_0005941, circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>_0086376 and circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>_0018168) were validated to have significant decrease in severe acne by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, Sanger sequencing and <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, in agreement with the results from <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010Seq data analysis. The five identified circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were predicted to interact with 213 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and regulated target gene expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study firstly showed that circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were differentially expressed in severe acne and suggested that circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s could be used as a potential biomarker for the drug targets of acne.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.14948","type":"journal-article","created":{"date-parts":[[2018,3,24]],"date-time":"2018-03-24T23:44:39Z","timestamp":1521935079000},"page":"1986-1992","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Circular <scp>RNA<\/scp> expression profile analysis of severe acne by <scp>RNA<\/scp>\u2010Seq and bioinformatics"],"prefix":"10.1111","volume":"32","author":[{"given":"J.","family":"Liang","sequence":"first","affiliation":[{"name":"Institute of Dermatology Guangzhou Medical University Guangzhou China"},{"name":"Department of Dermatology Guangzhou Institute of Dermatology Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Wu","sequence":"additional","affiliation":[{"name":"BGI Genomics BGI\u2010Shenzhen Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sun","sequence":"additional","affiliation":[{"name":"BGI Genomics BGI\u2010Shenzhen Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology Nanfang Hospital Southern Medical University Guangzhou 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Medical University Guangzhou China"},{"name":"Department of Dermatology Guangzhou Institute of Dermatology Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Dermatology Guangzhou Medical University Guangzhou China"},{"name":"Department of Dermatology Guangzhou Institute of Dermatology Guangzhou 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