{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:28:38Z","timestamp":1787498918885,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T00:00:00Z","timestamp":1526601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006393","name":"Universidad de Granada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006393","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["PI14\u201001024"],"award-info":[{"award-number":["PI14\u201001024"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2018,11]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Cutaneous leishmaniasis (\n CL<\/jats:styled-content>\n ) is a disfiguring and stigmatising disease occurring in more than 70 countries across the world including Spain and Morocco. The use of sensitive tests that can differentiate\n Leishmania s<\/jats:italic>\n pecies is advised.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n To evaluate the influence of the epidemiological scenario on the reliability of the\n PCR<\/jats:styled-content>\n techniques and contribute to the selection of the most efficient one for\n CL<\/jats:styled-content>\n diagnosis.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The sensitivities of parasitological methods and four\n PCR<\/jats:styled-content>\n s were compared in cutaneous samples from 77 patients from Spanish (\n PSH<\/jats:styled-content>\n ) and Moroccan hospitals (\n PMH<\/jats:styled-content>\n ). Exudates and fresh or paraffin\u2010embedded tissue biopsies were used.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n None of the\n PCR<\/jats:styled-content>\n s used in this study allowed the diagnosis of all\n CL<\/jats:styled-content>\n cases, showing also some drawbacks. Lmj4\/Uni21\u2010\n PCR<\/jats:styled-content>\n displayed the best sensitivity with\n PMH<\/jats:styled-content>\n , but it did not provide positive results in\n PSH<\/jats:styled-content>\n with\n CL<\/jats:styled-content>\n confirmed by other\n PCR<\/jats:styled-content>\n s. Conversely,\n JW<\/jats:styled-content>\n 13\/\n JW<\/jats:styled-content>\n 14\u2010\n PCR<\/jats:styled-content>\n and\n L. infantum\u2010<\/jats:italic>\n PCR<\/jats:styled-content>\n \u2010\n ELISA<\/jats:styled-content>\n displayed good sensitivities with\n PSH<\/jats:styled-content>\n that were not achieved with\n PMH<\/jats:styled-content>\n . Nested\u2010\n ITS<\/jats:styled-content>\n \u20101\u2010\n PCR<\/jats:styled-content>\n did not show enough sensitivity with paraffin\u2010embedded tissue biopsies. False\u2010negative results were obtained in 19% of\n PSH<\/jats:styled-content>\n due to unspecific hybridizations of\n ITS<\/jats:styled-content>\n \u20101 primers with human chromosome1.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n PCR<\/jats:styled-content>\n should be routinely used in patients with cutaneous lesions compatible with\n CL<\/jats:styled-content>\n and furthermore, the combination of two\n PCR<\/jats:styled-content>\n techniques is advisable. The selection of these\n PCR<\/jats:styled-content>\n s will be influenced by the epidemiological scenario: In areas where\n L. infantum<\/jats:italic>\n is endemic, the use of the\n PCR<\/jats:styled-content>\n \u2010\n ELISA<\/jats:styled-content>\n joint with\n JW<\/jats:styled-content>\n 13\/\n JW<\/jats:styled-content>\n 14\u2010\n PCR<\/jats:styled-content>\n seems an appropriate choice, whereas in areas such as Morocco, Lmj4\/Uni21 and\n ITS<\/jats:styled-content>\n \u20101 provide satisfactory results.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jdv.15034","type":"journal-article","created":{"date-parts":[[2018,4,28]],"date-time":"2018-04-28T04:44:30Z","timestamp":1524890670000},"page":"1999-2003","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Comparison of\n PCR<\/scp>\n \u2010based methods for the diagnosis of cutaneous leishmaniasis in two different epidemiological scenarios: Spain and Morocco"],"prefix":"10.1111","volume":"32","author":[{"given":"G.","family":"Merino\u2010Espinosa","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy Department of Parasitology University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Rodr\u00edguez\u2010Granger","sequence":"additional","affiliation":[{"name":"Department of Microbiology Complejo Hospitalario Universitario de Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Morillas\u2010M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Department of Parasitology University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Tercedor","sequence":"additional","affiliation":[{"name":"Microbiology, Internal Medicine and Dermatology Services Virgen de las Nieves Hospital Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Corpas\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Department of Parasitology University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Chiheb","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Ibn Rochd and Faculty of Medicine and Pharmacy University Hassan II of Casablanca Casablanca Morocco"},{"name":"Faculty of Medicine and Pharmacy Research team on Immunopathology of Infectious and Systemic Diseases University Hassan II of Casablanca Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Alcalde\u2010Alonso","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology Torrecardenas Hospital Almeria Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Aza\u00f1a\u2010Defez","sequence":"additional","affiliation":[{"name":"Department of Dermatology Complejo Hospitalario Universitario Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Riyad","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Pharmacy Research team on Immunopathology of Infectious and Systemic Diseases University Hassan II of Casablanca Casablanca Morocco"},{"name":"Laboratory of Parasitology\u2010Mycology Faculty of Medicine and Pharmacy University Hassan II of Casablanca Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"D\u00edaz\u2010S\u00e1ez","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Department of Parasitology University of Granada Granada 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