{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:29:01Z","timestamp":1790281741717,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,7,30]],"date-time":"2018-07-30T00:00:00Z","timestamp":1532908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Anti\u2010laminin\u2010\u03b31 (lam\u2010\u03b31) pemphigoid, a recently described immunobullous disorder sharing immune serological features of bullous pemphigoid and epidermolysis bullosa acquisita (<jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content>), is characterized by the detection of serum IgG autoantibodies against the lam\u2010\u03b31 chain, a 200 kDa heterotrimeric component of the dermal\u2010epidermal junction (<jats:styled-content style=\"fixed-case\">DEJ<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of the study was to develop an easy\u2010to\u2010perform and reliable assay for the serological detection of anti\u2010lam\u2010\u03b31 IgG autoantibodies. The clinical appearance alone is not sufficient to establish diagnosis of anti\u2010lam\u2010\u03b31 pemphigoid and rather requires immune serological evidence of (i) IgG reactivity against the dermal portion of salt\u2010split human skin; (ii) exclusion of IgG against other components of the <jats:styled-content style=\"fixed-case\">DEJ<\/jats:styled-content>; and (iii) IgG reactivity with a 200 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a protein of dermal extracts by immunoblot analysis (<jats:styled-content style=\"fixed-case\">IB<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The sera of 55 patients with anti\u2010lam\u2010\u03b31 pemphigoid were tested by <jats:styled-content style=\"fixed-case\">IB<\/jats:styled-content> with two recombinant heterotrimers, laminin 111 (lam\u2010111) and laminin 421 (lam\u2010421), as well as with a recombinant lam\u2010\u03b31 chain monomer. Additionally, a total of 41 control sera from patients with <jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content> (<jats:italic>n<\/jats:italic> = 15), psoriasis vulgaris (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>;<jats:italic> n<\/jats:italic> = 14), and healthy controls (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>;<jats:italic> n<\/jats:italic> = 12) were tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunoblot analysis revealed a positive reactivity with lam\u2010111 and\/or lam\u2010421 in 46\/55 (84%) of anti\u2010lam\u2010\u03b31 pemphigoid sera. Moreover, 8\/9 of the initially non\u2010reactive sera were positive with the lam\u2010\u03b31 monomer, leading to an overall sensitivity of 98.2%. Analyses of 41 control sera with the three lam\u2010\u03b31 recombinants led to a specificity of 88%. Specifically, 3\/15 <jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content> sera, 1\/14 <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> serum and 1\/12 <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content> serum reacted with the lam\u2010\u03b31 monomer while only the 3 <jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content> sera reacted with lam\u2010421.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Here we show a novel two\u2010step <jats:styled-content style=\"fixed-case\">IB<\/jats:styled-content> assay using the two recombinant laminin trimers and lam\u2010\u03b31 chain monomer for the detection of anti\u2010lam\u2010\u03b31 serum IgG with high sensitivity and specificity. This assay will facilitate the diagnosis and further characterization of this disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.15170","type":"journal-article","created":{"date-parts":[[2018,7,4]],"date-time":"2018-07-04T21:05:20Z","timestamp":1530738320000},"page":"735-741","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Diagnosis of anti\u2010laminin \u03b3\u20101 pemphigoid by immunoblot analysis"],"prefix":"10.1111","volume":"33","author":[{"given":"F.","family":"Solimani","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergology Philipps\u2010University Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Pollmann","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Philipps\u2010University Marburg 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