{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:21:54Z","timestamp":1792030914618,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T00:00:00Z","timestamp":1533600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Roskilde University doctoral school programme for Basic and Clinical Microbiology and Zealand University (former Roskilde) Hospital"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      An aberrant interaction between commensal skin bacteria and the host skin immune system is considered important in the pathogenesis of hidradenitis suppurativa (\n                      <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      In this study, we investigated the antibiotic susceptibility and biofilm\u2010forming capabilities of\n                      <jats:italic>S. epidermidis<\/jats:italic>\n                      strains isolated from\n                      <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>\n                      patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Skin biopsies were taken from active\n                      <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>\n                      lesions such as inflammatory nodules and\/or sinuses and non\u2010involved skin from 26 patients and cultured under optimal microbiological conditions for 24 h. Planktonic growth, biofilm production, antibiotic susceptibility and biofilm eradication by clindamycin, doxycycline, rifampicin and tetracycline were tested including a laboratory control strain of\n                      <jats:italic>S. epidermidis<\/jats:italic>\n                      for reference.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                      was cultured in 16 of 26\n                      <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>\n                      patients (62%). In total 27 different\n                      <jats:italic>S. epidermidis<\/jats:italic>\n                      isolates were identified; 16 (59%) from non\u2010involved skin and 11 (41%) from\n                      <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>\n                      lesions. All bacterial strains showed planktonic growth. Twenty\u2010four of 27 (89%) isolates were strong biofilm producers\n                      <jats:italic>in vitro<\/jats:italic>\n                      . The biofilm\u2010forming capability varied amongst the strains from non\u2010involved skin and lesional skin. Twenty\u2010four strains had an intermediate to resistant antibiotic susceptibility to clindamycin (89%). Rifampicin was the most effective antibiotic at inhibiting planktonic growth and at eradication of biofilm (\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      We observed a slight increase in\n                      <jats:italic>S. epidermidis<\/jats:italic>\n                      virulence, characterized by resistance to commonly used antibiotics, increased biofilm production and resistance to biofilm eradication. In particular, the reduced sensitivity to tetracycline and clindamycin, two standard antibiotics in the treatment of\n                      <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>\n                      , is alarming. Rifampicin, also important in\n                      <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>\n                      treatment, showed the greatest efficacy at eradicating the biofilm at low\n                      <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>\n                      concentrations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jdv.15183","type":"journal-article","created":{"date-parts":[[2018,7,19]],"date-time":"2018-07-19T05:29:45Z","timestamp":1531978185000},"page":"170-177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Biofilm production and antibiotic susceptibility of\n                    <i>Staphylococcus epidermidis<\/i>\n                    strains from Hidradenitis Suppurativa lesions"],"prefix":"10.1111","volume":"33","author":[{"given":"C.B.","family":"Ardon","sequence":"first","affiliation":[{"name":"Department of Dermatology Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands"},{"name":"Department of Science and Environment Roskilde University Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.P.","family":"Prens","sequence":"additional","affiliation":[{"name":"Department of Dermatology Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Fuursted","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection Control Staten Serum Institute Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.N.","family":"Ejaz","sequence":"additional","affiliation":[{"name":"Department of Science and Environment Roskilde University Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Shailes","sequence":"additional","affiliation":[{"name":"Department of Science and Environment Roskilde University Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Jenssen","sequence":"additional","affiliation":[{"name":"Department of Science and Environment Roskilde University Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.B.E.","family":"Jemec","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Zealand Roskilde Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1014163"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-2503"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12793"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2012.140064"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.182"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2015.08.010"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1999.02613.x"},{"key":"e_1_2_6_9_1","first-page":"3","article-title":"The skin microbiome","volume":"9","author":"Grice EA","year":"2011","journal-title":"Nat Rev Microbiol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)01563-0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2182"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02548.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-6701(99)90074-9"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.10.56"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.49.100195.003431"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02012-1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2012.00946.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15007"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-015-0516-5"},{"key":"e_1_2_6_20_1","unstructured":"The European Committee on Antimicrobial Susceptibility Testing.Breakpoint tables for interpretation of MICs and zone diameters version 8.0 2018. 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