{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:27:45Z","timestamp":1790659665989,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T00:00:00Z","timestamp":1557100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hidradenitis suppurativa (<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>) is a chronic inflammatory skin disease defined by recurrent nodules, tunnels and scarring involving the intertriginous regions. Recent next\u2010generation sequencing (<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>) studies suggest genera such as <jats:italic>Prevotella<\/jats:italic> spp., <jats:italic>Peptoniphilus<\/jats:italic> spp. and <jats:italic>Porphyromonas<\/jats:italic> spp. are associated with chronic and early <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> lesions. However, a systematic investigation of the bacterial microbiome in <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> tunnels remains unexplored using <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to investigate the bacterial composition of the luminal white gelatinous material found in <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> tunnels using <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An exploratory study of patients with diagnosis of <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a032) with tunnels. The tunnels were present either in the groin (<jats:italic>n<\/jats:italic>\u00a0=\u00a017) or in the axilla (<jats:italic>n<\/jats:italic>\u00a0=\u00a015). During deroofing of the tunnels, a sterile E\u2010swab was taken of the luminal gelatinous material. The samples were investigated using <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content> targeting 16S ribosomal <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The skin microbiome was characterized in 32 <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> patients. Overall, five microbiome types were identified: <jats:italic>Porphyromonas<\/jats:italic> spp. (type I), <jats:italic>Corynebacterium<\/jats:italic> spp., (type <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>), <jats:italic>Staphylococcus<\/jats:italic> spp. (type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>), <jats:italic>Prevotella<\/jats:italic> spp. (type <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>) and <jats:italic>Acinetobacter spp<\/jats:italic> (type V). <jats:italic>Porphyromonas<\/jats:italic> spp. (type I) and <jats:italic>Prevotella<\/jats:italic> spp. (<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>) were the most frequent genera found the tunnels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study points to a potential association between the presence of certain anaerobic bacteria (<jats:italic>Porphyromonas<\/jats:italic> spp., <jats:italic>Prevotella<\/jats:italic> spp.) and <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> tunnels. It may be speculated that these two genera are associated with the pathogenesis in <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> either as drivers or as biomarkers.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.15597","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T18:20:18Z","timestamp":1553538018000},"page":"1775-1780","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":96,"title":["The microbiome of tunnels in hidradenitis suppurativa patients"],"prefix":"10.1111","volume":"33","author":[{"given":"H.C.","family":"Ring","sequence":"first","affiliation":[{"name":"Department of Dermatology Zealand University Hospital Roskilde Denmark"},{"name":"Health Sciences Faculty University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Sigsgaard","sequence":"additional","affiliation":[{"name":"Department of Dermatology 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