{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T20:15:17Z","timestamp":1769112917293,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T00:00:00Z","timestamp":1562716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2016J01628"],"award-info":[{"award-number":["2016J01628"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although angiogenesis is an obvious pathological manifestation in the pathogenesis of syphilis, little is known about the underlying mechanisms of angiogenesis induced by reactions to <jats:italic>Treponema pallidum<\/jats:italic> antigens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>In this study, we sought to determine the role of recombinant <jats:italic>T.\u00a0pallidum<\/jats:italic> Tp47 in promoting angiogenesis in endothelial cells and the related mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Evaluation of the pro\u2010angiogenic activity of recombinant <jats:italic>T.\u00a0pallidum<\/jats:italic> Tp47 in human umbilical vein endothelial cells (HUVECs) was assessed, and the balance of matrix metalloproteinase (MMP)\/tissue inhibitor of metalloproteinase (TIMP) and the mechanisms underlying the involvement of Akt\/mTOR\/S6 pathways in this process were explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Under stimulation by Tp47, HUVECs exhibited obvious proliferation, migration and tube formation. In addition, the apparent promotion of angiogenesis by Tp47 was observed using a zebrafish embryo model. During angiogenesis, the levels of MMP\u20101 and MMP\u201010 were significantly elevated, whereas those of TIMP\u20101 and TIMP\u20102 did not change. In addition, after transfection with siRNAMMP\u20101 and siRNAMMP\u201010, migration and tube formation were significantly inhibited. Akt\/mTOR\/S6 signalling was found to be involved in upregulating MMP\u20101 and MMP\u201010 expression, and the sequential blockade of steps in the pathways effectively prevented Tp47\u2010induced angiogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results reveal the underlying mechanism of angiogenesis promoted by Tp47, namely, upregulating MMP\u20101 and MMP\u201010 expression to disrupt the MMP\/TIMP balance through the Akt\/mTOR\/S6 pathway. These findings contribute to our understanding of the pathophysiology of syphilis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.15725","type":"journal-article","created":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T12:33:44Z","timestamp":1559738024000},"page":"1958-1970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Recombinant <i>Treponema pallidum<\/i> protein Tp47 induces angiogenesis by modulating the matrix metalloproteinase\/tissue inhibitor of metalloproteinase balance in endothelial cells"],"prefix":"10.1111","volume":"33","author":[{"given":"Z.\u2010X.","family":"Gao","sequence":"first","affiliation":[{"name":"Center of Clinical Laboratory Zhongshan Hospital School of Medicine Xiamen University Xiamen China"},{"name":"Institute of Infectious Disease School of Medicine Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Luo","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Zhongshan Hospital School of Medicine Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.\u2010L.","family":"Liu","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Zhongshan Hospital School of Medicine Xiamen University Xiamen China"},{"name":"Institute of Infectious Disease School of Medicine Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.\u2010R.","family":"Lin","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Zhongshan Hospital School of Medicine Xiamen University Xiamen China"},{"name":"Institute of Infectious Disease School of Medicine Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.\u2010L.","family":"Tong","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Zhongshan Hospital School of Medicine Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4303-6252","authenticated-orcid":false,"given":"T.\u2010C.","family":"Yang","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Zhongshan Hospital School of Medicine Xiamen University Xiamen China"},{"name":"Institute of Infectious Disease School of Medicine Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu245"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu087"},{"key":"e_1_2_6_4_1","first-page":"143","article-title":"An electron microscopic study of a syphilitic chancre. 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