{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:54:36Z","timestamp":1791284076230,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T00:00:00Z","timestamp":1564444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T00:00:00Z","timestamp":1564444800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2020,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n The\n BIOCHIP<\/jats:styled-content>\n is a novel multiplex indirect immunofluorescence technique used in the serological diagnosis of bullous pemphigoid and pemphigus. The\n BIOCHIP<\/jats:styled-content>\n method combines the screening of autoantibodies and target antigen\u2010specific substrates in a single miniature incubation field.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n To evaluate the diagnostic accuracy of the new immunofluorescence\n BIOCHIP<\/jats:styled-content>\n multiplex tool in pemphigus and bullous pemphigoid.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n For the validation of the\n BIOCHIP<\/jats:styled-content>\n , sera from patients with\n BP<\/jats:styled-content>\n (\n n\u00a0<\/jats:italic>\n =\u00a038),\n PF<\/jats:styled-content>\n (\n n\u00a0<\/jats:italic>\n =\u00a08) and pemphigus vulgaris (\n PV<\/jats:styled-content>\n ) (\n n\u00a0<\/jats:italic>\n =\u00a023) were used. In addition, sera from disease control patients (\n n\u00a0<\/jats:italic>\n =\u00a063) and healthy volunteers (\n n\u00a0<\/jats:italic>\n =\u00a039) were used. The multiplex\n BIOCHIP<\/jats:styled-content>\n and direct immunofluorescence (\n DIF<\/jats:styled-content>\n ) were performed for all\n BP<\/jats:styled-content>\n ,\n PF<\/jats:styled-content>\n and\n PV<\/jats:styled-content>\n patients. Additional indirect immunofluorescence (IIF) was performed on patients with\n BP<\/jats:styled-content>\n , and\n ELISA<\/jats:styled-content>\n was performed on patients with pemphigus.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The\n BIOCHIP<\/jats:styled-content>\n mosaic showed a sensitivity of 86.8% and specificity of 85% for\n BP<\/jats:styled-content>\n 180 or\n BP<\/jats:styled-content>\n 230 being positive in\n BP<\/jats:styled-content>\n . It demonstrated a sensitivity of 75% and specificity of 97.7% for Dsg1 in\n PF<\/jats:styled-content>\n . The\n BIOCHIP<\/jats:styled-content>\n was found to have a sensitivity of 60.9% and specificity of 73.6% for Dsg3 in\n PV<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The\n BIOCHIP<\/jats:styled-content>\n mosaic\u2010based immunofluorescence test is potentially a simple, time and effort saving test that can aid in the diagnosis and screening of\n BP<\/jats:styled-content>\n ,\n PV<\/jats:styled-content>\n and\n PF<\/jats:styled-content>\n . However, there is potential for interpretation bias and a learning curve that needs to be taken into consideration.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jdv.15770","type":"journal-article","created":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T14:08:43Z","timestamp":1561990123000},"page":"153-160","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Validation of the\n BIOCHIP<\/scp>\n test for the diagnosis of bullous pemphigoid, pemphigus vulgaris and pemphigus foliaceus"],"prefix":"10.1111","volume":"34","author":[{"given":"A.","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Dermatology St George Hospital Sydney NSW Australia"},{"name":"Faculty of Medicine University of New South Wales Sydney NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Xuan","sequence":"additional","affiliation":[{"name":"Department of Dermatology St George 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