{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T05:42:38Z","timestamp":1792474958394,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2020,2]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Alopecia areata is a T\u2010cell\u2010mediated autoimmune disease with an unknown etiopathogenesis. Gut microbiota has been revealed as a key modulator of systemic immunity.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n To determine whether patients affected by alopecia universalis present differences in gut bacteria composition compared with healthy controls and investigate possible bacterial biomarkers of the disease.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We conducted a cross\u2010sectional study that involved 15 patients affected by alopecia universalis and 15 controls. Gut microbiome of the study subjects was analysed by sequencing the 16Sr\n RNA<\/jats:styled-content>\n of stool samples. We searched for bacterial biomarkers of alopecia universalis using the linear discriminant analysis effect size (\n LEF<\/jats:styled-content>\n se) tool.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n In total, 30 study subjects (46.6% female; mean [\n SD<\/jats:styled-content>\n ] age, 40.1 [9.8] years) were enrolled. Neither alpha (Shannon diversity index 5.31\u00a0\u00b1\u00a00.43 vs. 5.03\u00a0\u00b1\u00a00.43,\n P<\/jats:italic>\n 0.1) or beta diversity (\n ADONIS<\/jats:styled-content>\n P<\/jats:italic>\n value: 0.35) of gut microbiota showed statistically significant differences between cases and controls. In patients affected with alopecia, we found an enriched presence (\n LDA SCORE<\/jats:styled-content>\n \u00a0>\u00a02) of\n Holdemania filiformis, Erysipelotrichacea, Lachnospiraceae, Parabacteroides johnsonii, Clostridiales vadin<\/jats:italic>\n BB<\/jats:styled-content>\n 60 group,\n Bacteroides eggerthii<\/jats:italic>\n and\n Parabacteroides distasonis<\/jats:italic>\n . A predictive model based on the number of bacterial counts of\n Parabacteroides distasonis<\/jats:italic>\n and\n Clostridiales vadin<\/jats:italic>\n BB<\/jats:styled-content>\n 60 group correctly predicted disease status in 80% of patients (\n AUC<\/jats:styled-content>\n 0.804 (0.633\u20130.976),\n P<\/jats:italic>\n 0.004).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Alopecia universalis does not seem to affect broadly gut microbiota structure. Bacterial biomarkers found associated with the disease (\n \n Holdemania filiformis, Erysipelotrichacea, Lachnospiraceae, Parabacteroides johnsonii, Eggerthellaceae, Clostridiales vadin\n BB<\/jats:styled-content>\n 60 group, Bacteroides eggerthii\n <\/jats:italic>\n and\n Parabacteroides distasonis<\/jats:italic>\n ) should be further studied as they could be involved in its pathophysiology or be used as diagnostic tools.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jdv.15885","type":"journal-article","created":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T14:02:55Z","timestamp":1565964175000},"page":"400-405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":110,"title":["Analysis of the gut microbiota in alopecia areata: identification of bacterial biomarkers"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4850-8394","authenticated-orcid":false,"given":"O.M.","family":"Moreno\u2010Arrones","sequence":"first","affiliation":[{"name":"Dermatology Department Hospital Universitario Ram\u00f3n y Cajal IRYCIS, Universidad de Alcal\u00e1 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5447-3554","authenticated-orcid":false,"given":"S.","family":"Serrano\u2010Villar","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department Ramon y Cajal Hospital Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1927-9676","authenticated-orcid":false,"given":"V.","family":"Perez\u2010Brocal","sequence":"additional","affiliation":[{"name":"Department of Genomics and Health Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO\u2010Public Health) Val\u00e8ncia Spain"},{"name":"CIBER in Epidemiology and Public Health (CIBEResp) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6315-5462","authenticated-orcid":false,"given":"D.","family":"Saceda\u2010Corralo","sequence":"additional","affiliation":[{"name":"Dermatology Department Hospital Universitario Ram\u00f3n y Cajal IRYCIS, Universidad de Alcal\u00e1 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2453-8732","authenticated-orcid":false,"given":"C.","family":"Morales\u2010Raya","sequence":"additional","affiliation":[{"name":"Clinica Pedro Jaen Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3613-3844","authenticated-orcid":false,"given":"R.","family":"Rodrigues\u2010Barata","sequence":"additional","affiliation":[{"name":"Dermatology Department Hospital Universitario Ram\u00f3n y Cajal IRYCIS, Universidad de Alcal\u00e1 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1665-5389","authenticated-orcid":false,"given":"A.","family":"Moya","sequence":"additional","affiliation":[{"name":"Department of Genomics and Health Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO\u2010Public Health) Val\u00e8ncia Spain"},{"name":"CIBER in Epidemiology and Public Health (CIBEResp) Madrid Spain"},{"name":"Institute for Integrative Systems Biology (I2SysBio) The University of Valencia and The Spanish National Research Council (CSIC)\u2010UVEG) Val\u00e8ncia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7334-0044","authenticated-orcid":false,"given":"P.","family":"Jaen\u2010Olasolo","sequence":"additional","affiliation":[{"name":"Dermatology Department Hospital Universitario Ram\u00f3n y Cajal IRYCIS, Universidad de Alcal\u00e1 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2773-7494","authenticated-orcid":false,"given":"S.","family":"Vano\u2010Galvan","sequence":"additional","affiliation":[{"name":"Dermatology Department Hospital Universitario Ram\u00f3n y Cajal IRYCIS, Universidad de Alcal\u00e1 Madrid, 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