{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:10:57Z","timestamp":1787015457777,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,1,19]],"date-time":"2020-01-19T00:00:00Z","timestamp":1579392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2020,4]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Chancre self\u2010healing is an important clinical feature in the early stages of syphilis infection. Wound healing may involve an important mechanism by the migration of fibroblasts filling the injured lesion. However, the specific mechanism underlying this process is still unknown.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n We aimed to analyse the role of Tp0136 in the migration of fibroblasts and the related mechanism.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The migration ability of fibroblasts was detected by a wound\u2010healing assay.\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n and\n ELISA<\/jats:styled-content>\n detected the expression of\n MCP<\/jats:styled-content>\n \u20101,\n IL<\/jats:styled-content>\n \u20106 and\n MMP<\/jats:styled-content>\n \u20109.\n TLR<\/jats:styled-content>\n 4 expression was detected by\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n . The protein levels of\n CCR<\/jats:styled-content>\n 2 and relevant signalling pathway molecules were measured by Western blotting.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Tp0136 significantly promoted fibroblast migration. Subsequently, the levels of\n MCP<\/jats:styled-content>\n \u20101 and its receptor\n CCR<\/jats:styled-content>\n 2 were increased in this process. The migration of fibroblasts was significantly inhibited by an anti\u2010\n MCP<\/jats:styled-content>\n \u20101 neutralizing antibody or\n CCR<\/jats:styled-content>\n 2 inhibitors. Furthermore, studies demonstrated that Tp0136 could activate the\n ERK<\/jats:styled-content>\n \/\n JNK<\/jats:styled-content>\n \/\n PI<\/jats:styled-content>\n 3K\/\n NF<\/jats:styled-content>\n \u2010\u03baB signalling pathways through\n TLR<\/jats:styled-content>\n 4 activity and that signalling pathways inhibitors could weaken\n MCP<\/jats:styled-content>\n \u20101 secretion and fibroblast migration.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n These findings demonstrate that Tp0136 promotes the migration of fibroblasts by inducing\n MCP<\/jats:styled-content>\n \u20101\/\n CCR<\/jats:styled-content>\n 2 expression through signalling involving the\n TLR<\/jats:styled-content>\n 4,\n ERK<\/jats:styled-content>\n ,\n JNK<\/jats:styled-content>\n ,\n PI<\/jats:styled-content>\n 3K and\n NF<\/jats:styled-content>\n \u2010\u03baB signalling pathways, which could contribute to the mechanism of chancre self\u2010healing in syphilis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jdv.16162","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T12:01:38Z","timestamp":1576756898000},"page":"862-872","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Recombinant\n Treponema pallidum<\/i>\n protein Tp0136 promotes fibroblast migration by modulating\n MCP<\/scp>\n \u20101\/\n CCR<\/scp>\n 2 through\n TLR<\/scp>\n 4"],"prefix":"10.1111","volume":"34","author":[{"given":"X.","family":"Luo","sequence":"first","affiliation":[{"name":"Center of Clinical Laboratory Zhongshan Hospital School of Medicine Xiamen University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.\u2010X.","family":"Gao","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Zhongshan Hospital School of Medicine Xiamen University Xiamen 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