{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:02:39Z","timestamp":1791295359413,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001828","name":"Erasmus Universiteit Rotterdam","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001828","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      At the dermatology service of the General Hospital of Mexico City, Mexico, two patients, father and son, with black\u2010grain mycetoma were seen. The grains were isolated, and the cultured fungi were identified as\n                      <jats:italic>Madurella mycetomatis<\/jats:italic>\n                      based on morphology. Using the\n                      <jats:italic>M.\u00a0mycetomatis<\/jats:italic>\n                      specific PCR, amplicons of a different size than that of the\n                      <jats:italic>M.\u00a0mycetomatis<\/jats:italic>\n                      type strain were obtained.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the causative agent of the two black\u2010grain mycetoma cases and develop non\u2010culture\u2010based diagnostic tools to identify them to the species level.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>M.\u00a0mycetomatis<\/jats:italic>\n                      specific, the internal transcribed spacer (ITS) region, \u03b2\u2010tubulin (BT) and ribosomal binding protein 2 (RBP2) PCRs were used to confirm the identity of the isolates. Genetic variation was established by amplification fragment length polymorphisms. To determine the antifungal susceptibility profile, the Sensititre\u2122 YeastOne\u2122 assay was used. To develop a species\u2010specific PCR primers were designed on the sequenced PCR amplicon from the\n                      <jats:italic>M.\u00a0mycetomatis<\/jats:italic>\n                      specific PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      By analyzing the ITS, BT and RBP2 regions the isolates were identified as\n                      <jats:italic>Madurella pseudomycetomatis<\/jats:italic>\n                      . The isolates from father and son were similar but not identical to\n                      <jats:italic>M.\u00a0pseudomycetomatis<\/jats:italic>\n                      from Venezuela and one from an unknown origin.\n                      <jats:italic>Madurella pseudomycetomatis<\/jats:italic>\n                      isolates were inhibited by itraconazole, posaconazole and voriconazole but showed increased MIC values for amphotericin B and fluconazole. They were not inhibited by the echinocandins and five flucytosine. The two patients were treated with itraconazole resulting in cure for the father while the son was lost to follow\u2010up. The species\u2010specific PCR developed for\n                      <jats:italic>M.\u00a0pseudomyceotmatis<\/jats:italic>\n                      was discriminative and specific.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Madurella pseudomycetomatis<\/jats:italic>\n                      is genetically diverse with same susceptibility profile as\n                      <jats:italic>M.\u00a0mycetomatis<\/jats:italic>\n                      and causes eumycetoma in Latin America. The\n                      <jats:italic>M.\u00a0pseudomycetomatis<\/jats:italic>\n                      specific PCR can be used to identify this causative agent to the species level; however, this needs to be validated in an endemic setting.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jdv.16402","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T13:44:39Z","timestamp":1585662279000},"page":"1828-1834","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Diagnostic implications of mycetoma derived from\n                    <i>Madurella pseudomycetomatis<\/i>\n                    isolates from Mexico"],"prefix":"10.1111","volume":"34","author":[{"given":"B.","family":"Nyuykonge","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.H.W.","family":"Klaassen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.H.A.","family":"Zandijk","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.S.","family":"de Hoog","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.A.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute Utrecht The Netherlands"},{"name":"Faculty of Medical 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