{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T04:12:34Z","timestamp":1783829554405,"version":"3.55.0"},"reference-count":72,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T00:00:00Z","timestamp":1636416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Atopic dermatitis (AD) is an itchy, chronic and inflammatory skin condition, with dysfunctional immune response and skin barrier defects. Reduction of filaggrin (FLG) and tight junctions (TJ) proteins, such as claudin\u20101 (CLDN\u20101), expression in cutaneous epithelial barrier is remarkable in AD pathogenesis. Ocular involvement occurs in approximately 40% of AD patients leading to changes in the structure of the conjunctiva.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to evaluate the expression of FLG and CLDN\u20101 in the ocular surface of adults with AD, analysing bulbar conjunctival cells collected by a novel non\u2010invasive cellular imprint.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bulbar conjunctival epithelial cells were collected by cellular imprint technique, and FLG and CLDN\u20101 expression were assessed by immunofluorescence (IF) and real\u2010time polymerase chain reaction (RT\u2010PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We detected increased expression of FLG and CLDN\u20101, as well as their transcript levels in AD patients compared with healthy controls (HC). There was a positive correlation between tear film break\u2010up time (TBUT) and FLG expression. Fluorescein staining was inversely associated with FLG expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results may reflect a reactive response of the ocular surface to AD\u2010related ocular inflammation and associated dry eye disease. Further investigations focusing on the role of FLG and TJ expression in the ocular surface of AD patients may increment the understanding of the pathophysiology of extracutaneous AD and developing future targeted therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.17768","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T08:50:03Z","timestamp":1635324603000},"page":"247-254","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Increased expression of Filaggrin and Claudin\u20101 in the ocular surface of patients with atopic dermatitis"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4046-4531","authenticated-orcid":false,"given":"T.M.P.","family":"Callou","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Faculdade de Medicina FMUSP Universidade de S\u00e3o Paulo Sao Paulo 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