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The time\u2010consuming therapy includes daily baths and the application of skin care products to restore the epidermal barrier.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives and methods<\/jats:title><jats:p>To enhance the knowledge about the structure and function of the epidermal barrier in ichthyoses, we conducted clinical, biophysical and electron microscopical measurements on 46 patients with ichthyoses, including autosomal recessive ichthyoses, keratinopathic ichthyoses, X\u2010chromosomal\u2010recessive ichthyosis and Netherton syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The patients displayed a significantly decreased skin hydration along with unexpectedly low transepidermal waterloss values. Electron microscopical examinations demonstrated a severe occlusion of the epidermis by lipid remnants of skin care products in the stratum corneum. We found decreased intercellular lipid lamellae and an increased undulation of the corneocyte membrane of all ichthyoses, mostly pronounced in Netherton syndrome. The lipid profiles of ichthyoses showed decreased esterified \u03a9\u2010hydroxy\u2010sphingosine (EOS) ceramide levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results demonstrate the extent of the epidermal barrier disruption in ichthyoses. In combination with the knowledge about pathogenetic causes, individually improved therapeutic options can be derived from our results. In the future, the analyses of the organization of intercellular lipid lamellae and corneocyte membrane undulation will enable improved investigations of the epidermal barrier in ichthyoses and may be used to study and evaluate possible effects of topical skin preparations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.17914","type":"journal-article","created":{"date-parts":[[2022,1,11]],"date-time":"2022-01-11T18:29:38Z","timestamp":1641925778000},"page":"726-738","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of the structure and function of the epidermal barrier in patients with ichthyoses\u2014clinical and electron microscopical investigations"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9114-2351","authenticated-orcid":false,"given":"R.","family":"F\u00f6lster\u2010Holst","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venereology and 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