{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:39:00Z","timestamp":1791855540596,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T00:00:00Z","timestamp":1646870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The role of <jats:italic>Borrelia<\/jats:italic> in the development of skin lymphomas has been under discussion for decades. A similar association has been shown for <jats:italic>Helicobacter pylori<\/jats:italic> and gastric lymphomas (MALT type). Nevertheless, few molecular studies investigated <jats:italic>Borrelia<\/jats:italic> in skin lymphomas and the results are controversial.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analysed 46 formalin\u2010fixed, paraffin\u2010embedded skin specimens of clincopathologically confirmed B\u2010cell lymphomas (15 marginal zone lymphomas; 20 follicular lymphomas; three diffuse large B\u2010cell lymphomas; eight secondary cutaneous infiltrates) taken from 36 patients from Northern Germany, an endemic area for <jats:italic>Borrelia<\/jats:italic>. Fifteen pseudolymphomatous lesions of cutaneous Borreliosis served as the control. Both groups were examined with a real\u2010time (rt) PCR and a semi\u2010nested PCR targeting the 5S\u201023S intergenic spacer region (IGS). A multiplex PCR was used to investigate B\u2010cell clonality in all lymphomatous infiltrates (Biomed Primers).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>With both assays no <jats:italic>Borrelia burgdorferi<\/jats:italic>\u2010specific DNA was identified in any of the B\u2010cell lymphomas, while all 15 Borreliosis specimens gave a positive PCR result in the semi\u2010nested PCR protocol, 12 were also positive in the rt PCR (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). All B\u2010cell lymphomas showed monoclonal IgH\u2010Rearrangement. Analysis of cutaneous B\u2010cell lymphomas from available studies including ours (<jats:italic>n<\/jats:italic>\u2009=\u2009334) reveals an odds ratio &lt;1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>While some previous studies suggested an association between <jats:italic>B.\u2009burgdorferi<\/jats:italic> and the development of cutaneous B\u2010cell lymphomas in endemic areas, we were unable to confirm this in our patients, despite a highly sensitive <jats:italic>Borrelia<\/jats:italic> PCR assay. Our results including meta\u2010analysis of previous studies question the need for antibiotic therapy in patients with cutaneous B\u2010cell lymphomas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.18003","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T14:33:15Z","timestamp":1645021995000},"page":"836-845","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["No evidence of <i>Borrelia<\/i> in cutaneous infiltrates of B\u2010cell lymphomas with a highly sensitive, semi\u2010nested real\u2010time polymerase chain reaction targeting the 5S\u201023S intergenic spacer region"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1429-5159","authenticated-orcid":false,"given":"K.","family":"Papadopoulou","sequence":"first","affiliation":[{"name":"Dermatologikum Hamburg  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.M.","family":"Falk","sequence":"additional","affiliation":[{"name":"Dermatologikum Hamburg  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Metze","sequence":"additional","affiliation":[{"name":"Department of Dermatology M\u00fcnster University  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"B\u00f6er\u2010Auer","sequence":"additional","affiliation":[{"name":"Dermatologikum Hamburg  Hamburg Germany"},{"name":"Department of Dermatology M\u00fcnster University  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-04-152850"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-58012-7_3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25224"},{"key":"e_1_2_7_5_1","first-page":"6","article-title":"S2k \u2010 Leitlinie \u2010 Kutane Lymphome (ICD10 C82\u2013C86)","volume":"12","author":"Dippel E","year":"2021","journal-title":"AWMF Online"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190500305380"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-3679"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200009000-00012"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.1997.tb01318.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(97)80405-7"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(00)80233-6"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200308000-00003"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2011-0108"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0560.2001.281002.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-018-1376-2"},{"key":"e_1_2_7_16_1","first-page":"83","article-title":"Europe\u2010wide meta\u2010analysis of Borrelia burgdorferi sensu lato prevalence in questing Ixodes ricinus ticks","author":"Strnad M","year":"2017","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1085-5629(99)80010-8"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08670.x"},{"issue":"12","key":"e_1_2_7_19_1","article-title":"Diagnosis of Lyme borreliosis with a novel, semi\u2010nested real time Polymerase chain reaction targeting the 5S\u201323S intergenic spacer region: clinical features, histopathology and immunophenotype in 44 patients","volume":"43","author":"Podbicanin\u2010Ziburt A","year":"2021","journal-title":"Am J Dermatopathol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-11-881268"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-3-69"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-210x.2012.00250.x"},{"key":"e_1_2_7_24_1","unstructured":"Calculate Odds Ratio with 95% Confidence Intervals[WWW Document]. 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