{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T15:43:57Z","timestamp":1783957437693,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:00:00Z","timestamp":1647907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K12 CA090625"],"award-info":[{"award-number":["K12 CA090625"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["1.1.1.2\/VIAA\/4\/20\/665"],"award-info":[{"award-number":["1.1.1.2\/VIAA\/4\/20\/665"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013017","name":"Vanderbilt University Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013017","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["IK2 CX001785"],"award-info":[{"award-number":["IK2 CX001785"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The reliability to non\u2010invasively identify features of inflammatory dermatoses by reflectance confocal microscopy (RCM) remains unknown. Lack of formal training among RCM readers can result in inconsistent assessments, limiting clinical utility. Specific consensus terminology with representative images is necessary to ensure consistent feature\u2010level interpretation among RCM readers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>(1) Develop a glossary with representative images of RCM features of cutaneous acute graft\u2010versus\u2010host disease (aGVHD) for consistent interpretation among observers, (2) assess the interobserver reproducibility among RCM readers using the glossary, and (3) determine the concordance between RCM and histopathology for aGVHD features.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Through an iterative process of refinement and discussion among five international RCM experts, we developed a glossary with representative images of RCM features of aGVHD. From April to November 2018, patients suspected of aGVHD were imaged with RCM and subsequently biopsied. 17 lesions from 12 patients had clinically and pathologically confirmed cutaneous aGVHD. For each of these lesions, four dermatopathologists and four RCM readers independently evaluated the presence of aGVHD features in scanned histopathology slides and 1.5\u2009\u00d7\u20091.5\u2009mm RCM submosaics at 4 depths (blockstacks) respectively. RCM cases were adjudicated by a fifth RCM expert. Interobserver reproducibility was calculated by mean pairwise difference (U statistic). Concordance between modalities was determined by fraction of assignments with agreement.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We present a glossary with representative images of 18 aGVHD features by RCM. The average interobserver reproducibility among RCM readers (75%, confidence interval, CI: 71\u201379%) did not differ significantly from dermatopathologists (80%, 76\u201385%). The concordance between RCM and histopathology was 59%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>By using the glossary, the interobserver reproducibility among RCM readers was similar to the interobserver reproducibility among dermatopathologists. There was reasonable concordance between RCM and histopathology to visualize aGVHD features. The implementation of RCM can now be advanced in a variety of inflammatory conditions with a validated glossary and representative image set.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.18004","type":"journal-article","created":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T16:49:13Z","timestamp":1645116553000},"page":"1034-1044","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>In Vivo<\/i> reflectance confocal microscopy of cutaneous acute graft\u2010versus\u2010host disease: concordance with histopathology and interobserver reproducibility of a glossary with representative images"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4000-5485","authenticated-orcid":false,"given":"I.","family":"Saknite","sequence":"first","affiliation":[{"name":"Department of Dermatology Vanderbilt University Medical Center  Nashville TN USA"},{"name":"Biophotonics Laboratory Institute of Atomic Physics and Spectroscopy University of Latvia  Riga Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2413-1916","authenticated-orcid":false,"given":"M.","family":"Gill","sequence":"additional","affiliation":[{"name":"SkinMedical Research and Diagnostics PLLC  Dobbs Ferry NY USA"},{"name":"Department of Pathology SUNY Downstate Health Sciences University  Brooklyn NY USA"},{"name":"Faculty of Medicine and Health Sciences University of Alcal\u00e1  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6064-6311","authenticated-orcid":false,"given":"C.","family":"Alessi\u2010Fox","sequence":"additional","affiliation":[{"name":"Clinical Development Caliber Imaging and Diagnostics Inc  Rochester NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.P.","family":"Zwerner","sequence":"additional","affiliation":[{"name":"Department of Dermatology Vanderbilt 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