{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:46:24Z","timestamp":1791895584161,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T00:00:00Z","timestamp":1648080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1A2C2010276"],"award-info":[{"award-number":["2021R1A2C2010276"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1F1A1056138"],"award-info":[{"award-number":["2021R1F1A1056138"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Minocycline is a second\u2010generation tetracycline drug, which is widely used to treat diverse infectious and inflammatory diseases such as acne vulgaris. The effects of minocycline on acne vulgaris have been mainly attributed to its anti\u2010inflammatory effect; however, its sebum\u2010regulating effect and the relevance to epigenetic regulation in human sebaceous glands remain uninvestigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To identify the potential underlying epigenetic mechanism of sebum\u2010inhibitory effects of minocycline in human SZ95 sebocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The quantity of lipid droplets and the expression of key lipogenic genes were analysed in minocycline\u2010treated SZ95 sebocytes. To examine whether the sebum\u2010inhibitory effects of minocycline are relevant to histone acetylation, we analysed the effects of minocycline on p300 HAT and total HDAC activity. To elucidate the functional implication of p300 HAT inhibition by minocycline in sebocytes, we assessed the effect of p300 knockdown, inhibition and overexpression on lipid accumulation in SZ95 sebocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Minocycline suppressed the insulin and liver X receptor agonist\u2010induced lipid accumulation and the expression of the key lipogenic transcription factor sterol regulatory element\u2010binding protein 1 (SREBP1) and its downstream genes, fatty acid synthase (FAS) and acetyl\u2010CoA carboxylase \u03b1 (ACC\u03b1). Minocycline inhibited p300 HAT activity in a concentration\u2010dependent manner, but demonstrated no effect on global HDAC activity, resulting in a significant decrease in histone acetylation. Downregulation of p300 by knockdown or inhibition significantly suppressed SREBP1 expression, histone acetylation and lipid accumulation, whereas p300 overexpression enhanced these effects. Moreover, p300 overexpression rescued minocycline\u2010inhibited SREBP1 expression and lipid synthesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings revealed a novel sebum\u2010regulating effect of minocycline. Moreover, as p300 HAT is a key epigenetic regulator of sebaceous lipogenesis, its inhibitors could be used for the treatment of acne vulgaris.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.18079","type":"journal-article","created":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T08:30:17Z","timestamp":1647246617000},"page":"1325-1333","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Minocycline suppresses lipogenesis via inhibition of p300 histone acetyltransferase activity in human SZ95 sebocytes"],"prefix":"10.1111","volume":"36","author":[{"given":"H.S.","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Dermatology Seoul National University College of Medicine  Seoul Korea"},{"name":"Department of Biomedical Sciences Seoul National University Graduate School  Seoul Korea"},{"name":"Institute of Human\u2010Environment Interface Biology Medical Research Center Seoul National University  Seoul 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