{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T23:37:13Z","timestamp":1770421033845,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013996","name":"Global Health Innovative Technology Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013996","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Eumycetoma is a neglected tropical infection of the subcutaneous tissue commonly caused by the fungus <jats:italic>Madurella mycetomatis<\/jats:italic>. Previously, we demonstrated that \u03b2\u2010D\u2010glucan was present in the serum of eumycetoma patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare the performance of the recently approved easy\u2010to\u2010use Wako \u03b2\u2010D\u2010glucan assay to that of the Fungitell assay in eumycetoma patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using sera obtained from 41 eumycetoma, 12 actinomycetoma and 29 healthy endemic controls, we measured the \u03b2\u2010glucan serum concentrations using the Wako assay and compared the performance to that of the Fungitell assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>With the Fungitell assay, median \u03b2\u2010glucan serum concentrations of 208, 70 and 27\u2009pg\/ml were obtained for the 41 eumycetoma patients, the 12 actinomycetoma patients and the 29 healthy endemic controls, respectively. With the Wako assay these concentrations were 14.45, 11.57 and 2.5\u00a0pg\/ml, respectively. We demonstrated that when using the optimized cut\u2010off value (5.5\u00a0pg\/ml) for the Wako assay, the Wako and Fungitell assays had comparable performance in terms of sensitivity and specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The Wako assay is comparable to the Fungitell assay for measurement of serum \u03b2\u2010glucan in mycetoma patients and hence can be used in combination with current diagnostic tools. However, this test should be used in combination with other tests to differentiate actinomycetoma from eumycetoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.18642","type":"journal-article","created":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T16:54:04Z","timestamp":1665075244000},"page":"783-786","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Wako <scp>\u03b2\u2010D<\/scp>\u2010glucan assay can be used to measure serum <scp>\u03b2\u2010D<\/scp>\u2010glucan in Sudanese patients to aid with diagnosis of eumycetoma caused by <i>Madurella mycetomatis<\/i>"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1454-6149","authenticated-orcid":false,"given":"Bertrand","family":"Nyuykonge","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Erasmus MC University Medical Centre Rotterdam  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6314-7374","authenticated-orcid":false,"given":"Emmanuel","family":"Siddig","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Erasmus MC University Medical Centre Rotterdam  Rotterdam The Netherlands"},{"name":"Mycetoma Research Centre University of Khartoum  Khartoum Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Najwa A.","family":"Mhmoud","sequence":"additional","affiliation":[{"name":"Mycetoma Research Centre University of Khartoum  Khartoum Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahar","family":"Bakhiet","sequence":"additional","affiliation":[{"name":"Mycetoma Research Centre University of Khartoum  Khartoum Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduard","family":"Zijlstra","sequence":"additional","affiliation":[{"name":"Drugs for Neglected Diseases Initiative (DNDi)  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2242-8342","authenticated-orcid":false,"given":"Annelies","family":"Verbon","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Erasmus MC University Medical Centre Rotterdam  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4922-7321","authenticated-orcid":false,"given":"Ahmed H.","family":"Fahal","sequence":"additional","affiliation":[{"name":"Mycetoma Research Centre University of Khartoum  Khartoum Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8652-8783","authenticated-orcid":false,"given":"Wendy W. 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