{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:45:37Z","timestamp":1791895537784,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Acne is a multifactorial inflammatory disease of the pilosebaceous unit in which <jats:italic>Cutibacterium acnes<\/jats:italic> is one of the main triggers. A strong predominance of <jats:italic>C.\u00a0acnes<\/jats:italic> phylotype IA1 is present in acne skin with higher biofilm organization and virulence, promoting local immuno\u2010inflammation, especially the Th17 pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We evaluated the single and combined pharmacological properties of the plant extracts, <jats:italic>Myrtus communis<\/jats:italic> (Myrtacine\u00ae) and Celastrol enriched plant cell culture (CEE) extracts on the <jats:italic>C.\u00a0acnes<\/jats:italic>\/Th17 pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The effect of Myrtacine\u00ae on the virulence of <jats:italic>C.\u00a0acnes<\/jats:italic> phylotype IA1 was quantified according to the expression of several related genes. The activity of Myrtacine\u00ae and CEE on the inflammatory cascade was assessed using monocytes\u2010derived dendritic cells (Mo\u2010DC) stimulated with membranes or biofilms of the <jats:italic>C.\u00a0acnes<\/jats:italic> phylotype IA1. Finally, the effect of CEE on the Th17 pathway was studied using <jats:italic>C.\u00a0acnes<\/jats:italic> stimulated sebocyte 2D cultures and 3D skin tissue models containing preactivated Th17 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Myrtacine\u00ae had an anti\u2010virulence effect, evident as a significant and strong inhibition of the expression of several virulence factor genes by 60%\u201395% compared to untreated controls. Myrtacine\u00ae and CEE significantly inhibited proinflammatory cytokine (IL\u20106, IL\u20108, IL\u201012p40 and TNF\u2010\u03b1) production by Mo\u2010DC in response to <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>acnes<\/jats:italic> phylotype IA1. Interestingly, these two ingredients resulted in synergistic inhibition of most cytokines when used in combination. Finally, we demonstrated an inhibitory effect of CEE, in solution or formulated at 0.3%, specifically on IL\u201017 release by Th17 lymphocytes in a <jats:italic>C.\u00a0acnes<\/jats:italic>\u2010stimulated sebocyte 2D cultures and by Th17\u2010lymphocytes integrated in a 3D skin models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>2D and 3D models were developed to represent relevant and specific pathways involved in acne. Myrtacine\u00ae and CEE were shown to alter one or more of these pathways, indicating their potential beneficial effects on this disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.18793","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T16:43:41Z","timestamp":1675356221000},"page":"12-19","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>Myrtus communis<\/i> and Celastrol enriched plant cell culture extracts control together the pivotal role of <i>Cutibacterium acnes<\/i> and inflammatory pathways in acne"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8055-8255","authenticated-orcid":false,"given":"C\u00e9line","family":"Mias","sequence":"first","affiliation":[{"name":"Pierre Fabre Dermo\u2010Cosm\u00e9tique and Personal Care  Toulouse 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