{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T03:53:08Z","timestamp":1783655588954,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T00:00:00Z","timestamp":1693785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI17\/02017"],"award-info":[{"award-number":["PI17\/02017"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015756","name":"Sociedad Espa\u00f1ola de Enfermedades Infecciosas y Microbiolog\u00eda Cl\u00ednica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015756","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Whole\u2010genome sequencing (WGS) of <jats:italic>Neisseria gonorrhoeae<\/jats:italic> isolates combined with epidemiological and phenotypic data provides better understanding of population dynamics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The objective of this study was to investigate the molecular epidemiology of <jats:italic>N.\u2009gonorrhoeae<\/jats:italic> isolates from three centres in Spain and determine associations of antimicrobial resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Genetic characterization was performed in 170 <jats:italic>N.\u2009gonorrhoeae<\/jats:italic> isolates. WGS was carried out with the HiSeq platform (Illumina). Genome assemblies were submitted to the PubMLST Neisseria database website to determine NG\u2010MAST, MLST and NG\u2010STAR. Antimicrobial resistance genes and point mutations were identified with PubMLST. Phylogenomic comparison was based on whole\u2010genome single nucleotide polymorphism analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010six MLST, 49 NG\u2010MAST and 41 NG\u2010STAR sequence types were detected, the most prevalent being MLST\u2010ST9363 (27.1%), NG\u2010MAST ST569 (12.4%) and NG\u2010STAR ST193 (14.7%). Phylogenetic analysis identified 13 clusters comprising 69% of the isolates, with two of note: one involved cefixime\u2010resistant isolates from Barcelona presenting a mosaic <jats:italic>penA<\/jats:italic> X and belonging to MLST\u2010ST7363 and the other involved azithromycin\u2010resistant isolates from Mallorca that possessed the C2611T mutation in the four 23S rRNA alleles belonging to MLST\u2010ST1901.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The population of <jats:italic>N.\u2009gonorrhoeae<\/jats:italic> is quite heterogeneous in Spain. Our results agree with previous data published in Europe, albeit with some differences in distribution between regions. This study describes the circulation of two gonococcal populations with a specific resistance profile and sequence type in a specific geographic area. WGS is an effective tool for epidemiological surveillance of gonococcal infection and detection of resistance genes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.19458","type":"journal-article","created":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T02:48:54Z","timestamp":1692931734000},"page":"2575-2582","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genome\u2010based epidemiology and antimicrobial resistance of <i>Neisseria gonorrhoeae<\/i> in Spain: A prospective multicentre study"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2115-7413","authenticated-orcid":false,"given":"P.","family":"Salmer\u00f3n","sequence":"first","affiliation":[{"name":"Microbiology Department Vall d'Hebron Hospital Universitari, Vall d'Hebron Institut de Recerca (VHIR)  Barcelona Spain"},{"name":"Sexually Transmitted Infections Study Group (GEITS), Sociedad Espa\u00f1ola de Enfermedades Infecciosas y Microbiolog\u00eda Cl\u00ednica (SEIMC)  Madrid Spain"},{"name":"Department of Genetics and Microbiology Universitat Aut\u00f2noma de Barcelona (UAB)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Buckley","sequence":"additional","affiliation":[{"name":"University of Queensland, UQ Centre for Clinical Research (UQCCR)  Herston Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Arando","sequence":"additional","affiliation":[{"name":"Sexually Transmitted Infections Study Group (GEITS), Sociedad Espa\u00f1ola de Enfermedades Infecciosas y Microbiolog\u00eda Cl\u00ednica (SEIMC)  Madrid Spain"},{"name":"Drassanes\u2010Vall d'Hebron Sexually Transmitted Infections Unit Vall d'Hebron Hospital Universitari  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Alcoceba","sequence":"additional","affiliation":[{"name":"Microbiology Department Son Espases Hospital Universitari  Mallorca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Romero","sequence":"additional","affiliation":[{"name":"Microbiology Department, Ram\u00f3n y Cajal Hospital Universitario Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS)  Madrid Spain"},{"name":"CIBER of Epidemiology and Public Health (CIBERESP)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Clavo","sequence":"additional","affiliation":[{"name":"Sandoval Health Centre, San Carlos Hospital Cl\u00ednico Instituto de Investigaci\u00f3n Sanitaria San Carlos (IdISSC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Whiley","sequence":"additional","affiliation":[{"name":"University of Queensland, UQ Centre for Clinical Research (UQCCR)  Herston Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Serra\u2010Pladevall","sequence":"additional","affiliation":[{"name":"Sexually Transmitted Infections Study Group (GEITS), Sociedad Espa\u00f1ola de Enfermedades Infecciosas y Microbiolog\u00eda Cl\u00ednica (SEIMC)  Madrid Spain"},{"name":"Clinical Laboratory Hospital Universitari de Vic  Catalonia Spain"},{"name":"Faculty of Health Sciences Universitat de Vic \u2013 Universitat Central de Catalunya (UVIC \u2010UCC)  Vic Spain"},{"name":"Multidisciplinary Inflammation Research Group (MIRG) Fundaci\u00f3 Institut de Recerca i Innovaci\u00f3 en Ci\u00e8ncies de la Vida i de la Salut de la Catalunya Central  Vic Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,4]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"World Health Organization.Emergence of multi\u2010drug resistantNeisseria gonorrhoeae\u2013 Threat of global rise in untreatable sexually transmitted infections.2012; 2: 1. Available from:http:\/\/apps.who.int\/iris\/bitstream\/10665\/70603\/1\/WHO_RHR_11.14_eng.pdf. Accessed 25 February 2023."},{"key":"e_1_2_10_3_1","volume-title":"Annual epidemiological report for 2018","author":"ECDC. European Centre for Disease Prevention and Control","year":"2020"},{"key":"e_1_2_10_4_1","unstructured":"Unidad de vigilancia de VIH ITS y hepatitis B y C.Vigilancia Epidemiol\u00f3gica De Las Infecciones De Transmisi\u00f3n Sexual En Espa\u00f1a 2019. Centro Nacional de Epidemiolog\u00eda Instituto de Salud Carlos III\/Plan Nacional sobre el Sida Direcci\u00f3n General de Salud P\u00fablica; 2021. Available from:https:\/\/www.sanidad.gob.es\/ciudadanos\/enfLesiones\/enfTransmisibles\/sida\/vigilancia\/home.htm. Accessed 27 January 2023."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.117"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2807\/ese.15.47.19721\u2010en"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.2807\/ese.16.43.19998\u2010en"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05760-11"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00325-11"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2011.11.010"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1408109"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2402.171756"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0956462412472837"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2011.011267"},{"key":"e_1_2_10_15_1","unstructured":"Guidelines WHO.WHO guidelines for the treatment ofNeisseria gonorrhoeae. Available from:https:\/\/www.who.int\/publications\/i\/item\/9789241549691. Accessed 16 February 2023."},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.42.1900576"},{"key":"e_1_2_10_17_1","volume-title":"Gonococcal antimicrobial susceptibility surveillance in Europe \u2013 results summary 2017","author":"European Centre for Disease Prevention and Control","year":"2019"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1512757"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2018.23.27.1800323"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.8.1900118"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0956462419886775"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/0956462420949126"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6950a6"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medcli.2020.01.007"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2019.05.002"},{"key":"e_1_2_10_26_1","first-page":"114","article-title":"Typing and antimicrobial susceptibility of 134 Neisseria gonorrhoeae strains from southern Spain","volume":"32","author":"Cobo F","year":"2019","journal-title":"Rev Esp Quimioter"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30225-1"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03195-15"},{"key":"e_1_2_10_29_1","unstructured":"The European Committee on Antimicrobial Susceptibility Testing.Breakpoint tables for interpretation of MICs and zone diameters. Version 9.0 2019. Available from:http:\/\/www.eucast.org"},{"key":"e_1_2_10_30_1","unstructured":"AndrewsS.FastQC: a quality control tool for high throughput sequence data.2010Available from:http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2018.00198"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1196"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00100-17"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00044-1"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S095026881900150X"},{"key":"e_1_2_10_38_1","first-page":"25","article-title":"Epidemiolog\u00eda gen\u00f3mica y resistencia a antibi\u00f3ticos en gonococos en Espa\u00f1a","volume":"30","author":"Franc\u00e9s\u2010Cuesta C","year":"2018","journal-title":"Enferm Infecc Microbiol Clin"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab082"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa536"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2019.01.010"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000480"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab037"}],"container-title":["Journal of the European Academy of Dermatology and Venereology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jdv.19458","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,6]],"date-time":"2024-01-06T12:44:41Z","timestamp":1704545081000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jdv.19458"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,4]]},"references-count":42,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/jdv.19458"],"URL":"https:\/\/doi.org\/10.1111\/jdv.19458","archive":["Portico"],"relation":{},"ISSN":["0926-9959","1468-3083"],"issn-type":[{"value":"0926-9959","type":"print"},{"value":"1468-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,4]]},"assertion":[{"value":"2022-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}