{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:30:05Z","timestamp":1791847805055,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP18K08296"],"award-info":[{"award-number":["JP18K08296"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21K08344"],"award-info":[{"award-number":["JP21K08344"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Anti\u2010\u03b16\u03b24 integrin autoantibodies can be observed in some patients with mucous membrane pemphigoid. We have previously identified anti\u2010\u03b16\u03b24 integrin extracellular domain autoantibodies together with anti\u2010BP180 NC16A antibodies in a patient with DPP\u20104 inhibitor\u2010induced bullous pemphigoid. However, the significance and impact of anti\u2010\u03b16\u03b24 integrin antibodies are unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To characterize anti\u2010\u03b16\u03b24 integrin extracellular domain autoantibodies in pemphigoid patients, to determine whether these antibodies inhibit laminin\u2010\u03b16\u03b24 integrin binding and to observe their systemic effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Anti\u2010\u03b16\u03b24 integrin autoantibodies were analysed by staining cells expressing the extracellular region of \u03b16\u03b24 integrin with sera from 20 patients with pemphigoid. The anti\u2010\u03b16\u03b24 integrin autoantibodies were characterized using different transfectants. The binding of laminins to \u03b16\u03b24 integrin was studied using cells expressing the activated conformation of \u03b16\u03b24 integrin and the inhibitory effect of the autoantibodies on the binding of laminins to \u03b16\u03b24 integrin was tested. Trends in antibody titres and clinical symptoms were quantified and analysed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IgG autoantibodies against the extracellular domain of anti\u2010\u03b16\u03b24 integrin were found in some patients with pemphigoid. Laminin binding to \u03b16\u03b24 integrin was observed in the active conformation of \u03b16\u03b24 integrin, and serum from a patient with a high titre of anti\u2010\u03b16\u03b24 integrin antibodies inhibited the binding of both laminin\u2010511 and laminin\u2010332 to \u03b16\u03b24 integrin. \u03b16\u03b24 integrin is expressed on the basement membrane of both skin and small intestine, and exfoliation was observed in the patient's epidermis and small intestinal epithelium. A reduction in the titre of the anti\u2010\u03b16\u03b24 integrin antibody was associated with improvement in both skin and gastrointestinal symptoms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrated the presence of anti\u2010\u03b16\u03b24 integrin extracellular domain\u2010specific autoantibodies in some patients with pemphigoid. In addition, these autoantibodies showed inhibitory activity on \u03b16\u03b24 integrin\u2010laminin binding. Anti\u2010\u03b16\u03b24 integrin antibodies can affect the gastrointestinal tract as well as the skin and oral mucosa.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.19582","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T03:01:30Z","timestamp":1697770890000},"page":"404-412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti\u2010\u03b16\u03b24 integrin autoantibodies inhibit the binding of laminins to \u03b16\u03b24 integrin in patients with pemphigoid and affect the gastrointestinal tract"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3966-3437","authenticated-orcid":false,"given":"Noriko","family":"Arase","sequence":"first","affiliation":[{"name":"Department of Dermatology Osaka University Graduate School of Medicine  Osaka 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