{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:21:40Z","timestamp":1784892100759,"version":"3.55.0"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:00:00Z","timestamp":1709164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1F1A1074674"],"award-info":[{"award-number":["2020R1F1A1074674"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2022M3A9D3016848"],"award-info":[{"award-number":["2022M3A9D3016848"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Studies on the interaction between tumour\u2010infiltrating immune cells (TIICs) and tumour cells in melanoma arising from congenital melanocytic nevus (CMN) are lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to determine the intratumoral immune landscape of TIICs and tumour cells during invasion and metastasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tissue specimens were obtained from patients with melanoma originating from CMN. Differential gene expression in melanoma cells and TIICs during invasion and metastasis was determined using spatial transcriptomics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As invasion depth increased, the expression of <jats:italic>LGALS3<\/jats:italic>, known to induce tumour\u2010driven immunosuppression, increased in melanoma cells. In T cells, the expression of genes that inhibit T\u2010cell activation increased with increasing invasion depth. In macrophages, the expression of genes related to the anti\u2010inflammatory M2 phenotype was upregulated with increasing invasion depth. Compared to primary tumour cells, melanoma cells in metastatic lesions showed upregulated expression of genes associated with cancer immune evasion, including <jats:italic>AXL<\/jats:italic> and <jats:italic>EPHA2<\/jats:italic>, which impede T\u2010cell recruitment, and <jats:italic>BST2<\/jats:italic>, associated with M2 polarization. Furthermore, T cells showed increased expression of genes related to immunosuppression, and macrophages exhibited increased expression of genes associated with the M2 phenotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The interaction between melanomas arising from CMN and TIICs may be important for tumour progression and metastasis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.19881","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T09:30:23Z","timestamp":1709199023000},"page":"1599-1605","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Spatial transcriptomic analysis of tumour\u2013immune cell interactions in melanoma arising from congenital melanocytic nevus"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6409-2704","authenticated-orcid":false,"given":"Youngkyoung","family":"Lim","sequence":"first","affiliation":[{"name":"Department of Dermatology Veterans Health Service Medical Center  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beom Keun","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Seoul National University Graduate School  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong\u2010Jun","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Seoul National University Graduate School  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soyoung","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Seoul National University Graduate School  Seoul 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