{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:54:13Z","timestamp":1792176853441,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T00:00:00Z","timestamp":1724025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013126","name":"Psoriasis Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013126","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Eczema and psoriasis are common diseases. Despite both showing active epidermal contribution to the inflammatory process, their molecular aetiology and pathological mechanisms are different.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Further molecular insight into these differences is therefore needed to enable effective future diagnostic and treatment strategies. The majority of our mechanistic and clinical understanding of psoriasis and eczema is derived from RNA, immunohistology and whole skin biopsy data.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, non\u2010invasive epidermal sampling of lesional, perilesional and non\u2010lesional skin from diseased and healthy skin was used to perform an in depth proteomic analysis of epidermal proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our findings confirmed the psoriasis\u2010associated cytokine IL\u201036\u03b3 as an excellent protein biomarker for lesional psoriasis. However, ELISA and ROC curve analysis of 53 psoriasis and 42 eczema derived samples showed that the sensitivity and specificity were outperformed by elastase\u2010specific protease inhibitor, elafin. Of note, elafin was also found upregulated in non\u2010lesional psoriatic skin at non\u2010predilection sites demonstrating inherent differences between the non\u2010involved skin of healthy and psoriatic individuals. Mass spectrometry and ELISA analysis also demonstrated the upregulation of the anti\u2010inflammatory molecule IL\u201037 in psoriatic perilesional but not lesional skin. The high expression of IL\u201037 surrounding psoriatic plaque may contribute to the sharp demarcation of inflammatory morphology changes observed in psoriasis. This finding was also specific for psoriasis and not seen in atopic dermatitis or autoimmune blistering perilesional skin. Our results confirm IL\u201036\u03b3 and add elafin as robust, hallmark molecules distinguishing psoriasis and eczema\u2010associated inflammation even in patients under systemic treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Overall, these findings highlight the potential of epidermal non\u2010invasive sampling and proteomic analysis to increase our diagnostic and pathophysiologic understanding of skin diseases. Moreover, the identification of molecular differences in healthy\u2010looking skin between patients and healthy controls highlights potential disease susceptibility markers and proteins involved in the initial stages of disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jdv.20289","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T05:39:10Z","timestamp":1724045950000},"page":"1324-1335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Epidermal proteomics demonstrates Elafin as a psoriasis\u2010specific biomarker and highlights increased anti\u2010inflammatory activity around psoriatic plaques"],"prefix":"10.1111","volume":"39","author":[{"given":"Anna","family":"Berekmeri","sequence":"first","affiliation":[{"name":"Leeds Institute of Rheumatic and Musculoskeletal Medicine (LIRMM) University of Leeds  Leeds UK"},{"name":"National Institute for Health Research (NIHR) Leeds Biomedical Research Centre (BRC) The Leeds Teaching Hospitals  Leeds UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Macleod","sequence":"additional","affiliation":[{"name":"Leeds Institute of Rheumatic and Musculoskeletal Medicine (LIRMM) University of Leeds  Leeds UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Hyde","sequence":"additional","affiliation":[{"name":"Leeds Institute of Rheumatic and Musculoskeletal Medicine (LIRMM) University of Leeds  Leeds UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1675-186X","authenticated-orcid":false,"given":"Gregor Jan","family":"Ojak","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Medical Centre Johannes Gutenberg\u2010University Mainz  Mainz 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