{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:29:50Z","timestamp":1792189790423,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018703","name":"HORIZON EUROPE European Innovation Council","doi-asserted-by":"publisher","award":["101046667"],"award-info":[{"award-number":["101046667"]}],"id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012818","name":"Comunidad de Madrid","doi-asserted-by":"publisher","award":["2020\u2010T1\/TIC\u201020661TEC"],"award-info":[{"award-number":["2020\u2010T1\/TIC\u201020661TEC"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["03VP102070"],"award-info":[{"award-number":["03VP102070"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["862811"],"award-info":[{"award-number":["862811"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["871763"],"award-info":[{"award-number":["871763"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Efficient staging and management of cutaneous melanoma, one of today's deadliest skin cancers, requires non\u2010invasive determination of tumour depth (Breslow depth). However, current imaging technologies lack the necessary contrast or penetration to measure Breslow depth.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine if raster\u2010scanning optoacoustic mesoscopy (RSOM) can fill this gap in dermatology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used phantoms to optimize RSOM for melanoma imaging and demonstrated its capability to image melanocytes at single\u2010cell resolution in deep tissue. We then compared RSOM's ability to measure Breslow depth against the clinical standard in a pilot study.<\/jats:p>\n                    <jats:p>\n                      For the phantoms studies, we compared the ability of an optimized RSOM system to measure the concentration and diameter of single melanoma cells against gold\u2010standard microscopy methods. For the pilot clinical study, we used linear regression to compare RSOM's Breslow depth against the clinical standard, obtaining the goodness of fit (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      ) and the\n                      <jats:italic>p<\/jats:italic>\n                      \u2010value.\n                    <\/jats:p>\n                    <jats:p>For the pilot clinical study, we imaged nine lesions: 7 superficially spreading melanomas, 1 benign dysplastic nevus and 1 blue nevus. The average age of the patients was 56.2\u2009\u00b1\u200912.5\u2009years. We also imaged 10 non\u2010lesional skin areas from healthy volunteers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      By utilizing ultra\u2010wideband frequency detection and optimized illumination wavelength, we show that RSOM achieves non\u2010invasive imaging of melanoma at the resolution of single melanocytes, penetrating more than 3\u2009mm into the skin. The agreement between RSOM and the standard\u2010of\u2010care histological assessment was\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20090.886 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0002) for Breslow depth determination.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>RSOM provides non\u2010invasive imaging performance that correlates with the Breslow depth determination. Further work is needed to confirm these findings and to test RSOM against other non\u2010invasive methods.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jdv.20811","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T09:29:26Z","timestamp":1751534966000},"page":"1945-1954","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Non\u2010invasive characterization of melanoma depth at single\u2010cell resolution"],"prefix":"10.1111","volume":"39","author":[{"given":"Juan","family":"Aguirre","sequence":"first","affiliation":[{"name":"Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health &amp; School of Computation, Information and Technology Technical University of Munich  Munich Germany"},{"name":"Institute of Biological and Medical Imaging, Bioengineering Center Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"},{"name":"Departamento de Tecnolog\u00eda Electr\u00f3nica y de las Comunicaciones Universidad Autonoma de Madrid  Madrid Spain"},{"name":"Instituto de Investigacion Sanitaria de la Fundacion Jimenez Diaz  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Gasteiger","sequence":"additional","affiliation":[{"name":"Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health &amp; School of Computation, Information and Technology Technical University of Munich  Munich Germany"},{"name":"Institute of Biological and Medical Imaging, Bioengineering Center Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"},{"name":"Department of Dermatology and Allergology Technical University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4350-0518","authenticated-orcid":false,"given":"Benedikt","family":"Hindelang","sequence":"additional","affiliation":[{"name":"Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health &amp; School of Computation, Information and Technology Technical University of Munich  Munich Germany"},{"name":"Institute of Biological and Medical Imaging, Bioengineering Center Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"},{"name":"Department of Dermatology and Allergology Technical University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Seeger","sequence":"additional","affiliation":[{"name":"Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health &amp; School of Computation, Information and Technology Technical University of Munich  Munich Germany"},{"name":"Institute of Biological and Medical Imaging, Bioengineering Center Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei","family":"Bereznhoi","sequence":"additional","affiliation":[{"name":"Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health &amp; School of Computation, Information and Technology Technical University of Munich  Munich Germany"},{"name":"Institute of Biological and Medical Imaging, Bioengineering Center Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"Weidenfeld","sequence":"additional","affiliation":[{"name":"Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health &amp; School of Computation, Information and Technology Technical University of Munich  Munich Germany"},{"name":"Institute of Biological and Medical Imaging, Bioengineering Center Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf","family":"Darsow","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Technical University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre C.","family":"Stiel","sequence":"additional","affiliation":[{"name":"Institute of Biological and Medical Imaging, Bioengineering Center Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"},{"name":"Protein Engineering for Superresolution Microscopy Lab University of Regensburg  Regensburg Bavaria Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne Annette","family":"Steimle\u2010Grauer","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Technical University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0296-3567","authenticated-orcid":false,"given":"Christian","family":"Posch","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Technical University of Munich  Munich Germany"},{"name":"Department for Dermatology Vienna Healthcare Group, Clinic Hietzing  Vienna Austria"},{"name":"Sigmund Freud University Vienna Faculty of Medicine  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilo","family":"Biedermann","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Technical University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9988-0233","authenticated-orcid":false,"given":"Vasilis","family":"Ntziachristos","sequence":"additional","affiliation":[{"name":"Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health &amp; 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