{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:32:54Z","timestamp":1769506374602,"version":"3.49.0"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31821001"],"award-info":[{"award-number":["31821001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31901223"],"award-info":[{"award-number":["31901223"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170528"],"award-info":[{"award-number":["32170528"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Evolutionary Biology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thickness reduction or loss of the calcareous eggshell is one of major phenotypic changes in the transition from oviparity to viviparity. Whether the reduction of eggshells in viviparous squamates is associated with specific gene losses is unknown. Taking advantage of a newly generated high\u2010quality genome of the viviparous Chinese crocodile lizard (<jats:italic>Shinisaurus crocodilurus<\/jats:italic>), we found that ovocleidin\u201017 gene (<jats:italic>OC\u201017<\/jats:italic>), which encodes an eggshell matrix protein that is essential for calcium deposition in eggshells, is not intact in the crocodile lizard genome. Only <jats:italic>OC\u201017<\/jats:italic> transcript fragments were found in the oviduct transcriptome, and no OC\u201017 peptides were identified in the eggshell proteome of crocodile lizards. In contrast, OC\u201017 was present in the eggshells of the oviparous Mongolia racerunner (<jats:italic>Eremias argus<\/jats:italic>). Although the loss of <jats:italic>OC\u201017<\/jats:italic> is not common in viviparous species, viviparous squamates show fewer intact eggshell\u2010specific proteins than oviparous squamates. Our study implies that functional loss of eggshell\u2010matrix protein genes may be involved in the reduction of eggshells during the transition from oviparity to viviparity in the crocodile lizard.<\/jats:p>","DOI":"10.1111\/jeb.14097","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T17:28:34Z","timestamp":1663781314000},"page":"1568-1575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The eggshell\u2010matrix protein gene <i><scp>OC<\/scp>\u201017<\/i> is functionally lost in the viviparous Chinese crocodile lizard"],"prefix":"10.1093","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8012-6575","authenticated-orcid":false,"given":"Hong\u2010Xin","family":"Xie","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi\u2010Xi","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Ming","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Qiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Novogene Bioinformatics Institute  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi\u2010Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi\u2010Han","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu\u2010Ming","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2275-2591","authenticated-orcid":false,"given":"Wei\u2010Guo","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.39"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10390"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0036-z"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq662"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1865504"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/jtbi.1995.0092"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1655\/0733-1347(2007)20[131:SRAMOF]2.0.CO;2"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm6494"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz297"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-814067-3.00010-7"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2008.01794.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.15409"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201000679"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp200145m"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/gix041"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1816086116"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104543200"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610294200"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1883"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evw229"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1365-2656.13475"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1818504116"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008200390152025"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.46.32915"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00298593"},{"key":"e_1_2_8_28_1","first-page":"287","article-title":"Present status of Chinese xenosaurs and its protection","volume":"30","author":"Huang W.","year":"2009","journal-title":"Chinese Journal of Wildlife"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaw6671"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx116"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.072658899"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/zoo.21473"},{"key":"e_1_2_8_33_1","doi-asserted-by":"crossref","unstructured":"Li W.\u2010M. Du J. Yang L.\u2010Y. Liang Q.\u2010Q. Yang M.\u2010Y. Zhou X.\u2010M. &Du W.\u2010G.(2022).Chromosome\u2010level genome assembly and population genomics of Mongolian racerunner (Eremias argus) provide insights into high\u2010altitude adaptation in lizards. Under review.","DOI":"10.1186\/s12915-023-01535-z"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12953-015-0086-1"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12953-015-0078-1"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2003.09.006"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2014.09.024"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf020597x"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-011-0584-0"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1086\/302219"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1998.tb02265.x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.095"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-021-01555-4"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406033200"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0046-y"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1107\/S139900471500125X"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr109"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10877"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz828"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2010.02034.x"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.136"},{"key":"e_1_2_8_53_1","first-page":"19","article-title":"Last chance to see? A review of the threats to and use of the crocodile lizard","volume":"27","author":"Schingen M.","year":"2015","journal-title":"Traffic Bulletin"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky427"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.01.043"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.22642"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab359"},{"key":"e_1_2_8_58_1","first-page":"1164","article-title":"Research advances in ovocleidin\u201017, the eggshell\u2010specific matrix protein from avians","volume":"025","author":"Yu D.","year":"2013","journal-title":"Chinese Journal of Animal Nutrition"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2015.10.009"}],"container-title":["Journal of Evolutionary Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jeb.14097","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jeb.14097","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jeb.14097","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T20:32:38Z","timestamp":1706041958000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jeb\/article\/35\/11\/1568-1575\/7317949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,21]]},"references-count":58,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/jeb.14097"],"URL":"https:\/\/doi.org\/10.1111\/jeb.14097","archive":["Portico"],"relation":{},"ISSN":["1010-061X","1420-9101"],"issn-type":[{"value":"1010-061X","type":"print"},{"value":"1420-9101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,21]]},"assertion":[{"value":"2021-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}