{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:07:49Z","timestamp":1784167669508,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:00:00Z","timestamp":1656374400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Evidence Based Medicine"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to compare the accuracy of QuantiFERON\u2010TB Gold Plus (QFT\u2010Plus) and T\u2010SPOT.TB for diagnosing active tuberculosis (ATB) in febrile patients, to explore influencing factors of positive results and to verify the potential value of QFT\u2010Plus in the identification of ATB and latent tuberculosis infection (LTBI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 240 febrile patients with ATB (<jats:italic>n<\/jats:italic> = 80) and non\u2010ATB (<jats:italic>n<\/jats:italic> = 160) were recruited to assess the accuracy of QFT\u2010Plus and T\u2010SPOT.TB for diagnosing ATB. Multivariable logistic regression was used to analyze the influencing factors of positive results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The proportion of indeterminate results (ITRS) in QFT\u2010Plus and T\u2010SPOT.TB were 3.3% and 0%, respectively. The consistency between the results of the QFT\u2010Plus and T\u2010SPOT.TB was substantial. The area under the receiver operating characteristic curve (AUROC) of the QFT\u2010Plus and T\u2010SPOT.TB for diagnosing ATB was 0.792 and 0.849 (<jats:italic>p =<\/jats:italic> 0.070), respectively. The sensitivity of differentiating ATB from non\u2010ATB was 92.2% in QFT\u2010Plus versus 95.0% in T\u2010SPOT.TB. The influencing factors of T\u2010SPOT.TB positive result were male (odds ratio (OR) = 2.33, 95% confidence interval (CI) 1.27\u20134.26, <jats:italic>p =<\/jats:italic> 0.006), evidence of previous TB (OR 11.36, 95% CI 4.62\u201327.94, <jats:italic>p &lt;<\/jats:italic> 0.001), while male (OR = 3.17, 95% CI 1.73\u20135.84, <jats:italic>p &lt;<\/jats:italic> 0.001), evidence of previous TB (OR = 7.58, 95% CI 3.60\u201315.98, <jats:italic>p &lt;<\/jats:italic>0.001), and use of immunosuppressant (OR = 0.49, 95% CI 0.260.94, <jats:italic>p<\/jats:italic> = 0.030) were influencing factors for QFT\u2010Plus positive result. There was no significant difference in QFT\u2010Plus in differentiating ATB from LTBI in febrile patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>There was no significant difference between QFT\u2010Plus and T\u2010SPOT.TB for diagnosing ATB in febrile patients. QFT\u2010Plus is prone to ITRS. The influencing factors including males, evidence of the previous TB, and use of immunosuppressant should be considered when interpreting positive results.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jebm.12477","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T11:08:50Z","timestamp":1656414530000},"page":"97-105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparison of diagnostic accuracy of QuantiFERON\u2010TB Gold Plus and T\u2010SPOT.TB in the diagnosis of active tuberculosis in febrile patients"],"prefix":"10.1111","volume":"15","author":[{"given":"Lifan","family":"Zhang","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal medicine, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"},{"name":"Clinical 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Internal medicine, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiping","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Beijing Chest Hospital Capital Medical University\/Beijing Tuberculosis and Thoracic Tumor Research Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueqiu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal medicine, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 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