{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T07:42:48Z","timestamp":1775893368755,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["304444\/2025\u20101"],"award-info":[{"award-number":["304444\/2025\u20101"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","award":["11221070"],"award-info":[{"award-number":["11221070"]}],"id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","award":["1170575"],"award-info":[{"award-number":["1170575"]}],"id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Esthet Restor Dent"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the effect of adding zinc oxide and copper nanoparticles (ZnO\/CuNp) into universal adhesives (UAs) on resin\u2013dentin microtensile bond strength (\u03bcTBS), nanoleakage (NL), and presence of ZnO\/Cu within the hybrid layer on caries\u2010affected dentin, both at baseline (T0) and after 2\u2009years of water storage (T2).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      ZnO\/CuNp (0% [control]; 5\/0.1 and 5\/0.2\u2009wt%) were added into Prime&amp;Bond Active (PBA) and Ambar Universal (AMB). Ninety\u2010six extracted human third molars were used. After microbiological induction of caries\u2010affected dentin, UAs were applied in both etch\u2010and\u2010rinse and self\u2010etch modes, followed by composite build\u2010up. Specimens were sectioned to obtain resin\u2013caries\u2010affected dentin bonded sticks for \u03bcTBS, NL, and chemical characterization of the hybrid layer for Cu and Zn detection at T0 and T2. Data were statistically analyzed (\n                      <jats:italic>\u03b1<\/jats:italic>\n                      \u2009=\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ZnO\/CuNp had no effect on \u03bcTBS at T0 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05) but improved \u03bcTBS at T2, compared to control (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Both UAs showed a significant decrease in \u03bcTBS over time (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), except PBA with ZnO\/CuNp in etch\u2010and\u2010rinse mode. NL was significantly lower in ZnO\/CuNp\u2010containing UAs at both time points (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), though all groups showed increased NL over time. ZnO\/CuNp remained detectable in the hybrid layer at T2.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Adding ZnO\/CuNp to UAs decreased adhesive interface degradation on caries\u2010affected dentin, likely due to their presence within the hybrid layer even after 2\u2009years.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Significance<\/jats:title>\n                    <jats:p>ZnO\/Cu nanoparticles into UAs could enhance the stability of adhesive restorations on caries\u2010affected dentin by reducing interface degradation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jerd.70095","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T17:28:12Z","timestamp":1767115692000},"page":"970-980","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Universal Dental Adhesives Containing Zinc Oxide and Copper Nanoparticles Improve Interface on Caries\u2010Affected Dentin After 2\u2009Years: In\u00a0Vitro Study"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1827-5956","authenticated-orcid":false,"given":"Mario Felipe","family":"Guti\u00e9rrez","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n e Innovaci\u00f3n Biom\u00e9dica (CIIB) Universidad de los Andes, Chile. Facultad de Odontolog\u00eda  Santiago Chile"},{"name":"Department of Restorative Dentistry, Faculty of Dentistry University of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romina","family":"Aliaga\u2010G\u00e1lvez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n e Innovaci\u00f3n Biom\u00e9dica (CIIB) Universidad de los Andes, Chile. Facultad de Odontolog\u00eda  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romina","family":"\u00d1aupari\u2010Villasante","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, School of Dentistry State University of Ponta Grossa  Ponta Grossa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3594-4648","authenticated-orcid":false,"given":"Eduardo","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Faculty of Dentistry University of Chile  Santiago Chile"},{"name":"Instituto de Ciencias Biom\u00e9dicas, Universidad Aut\u00f3noma de Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9880-4856","authenticated-orcid":false,"given":"Alessandro D.","family":"Loguercio","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, School of Dentistry State University of Ponta Grossa  Ponta Grossa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"7","article-title":"Bonding to Sound and Caries\u2010Affected Dentin: A Systematic Review and Meta\u2010Analysis","volume":"20","author":"Isolan C. 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