{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:13:58Z","timestamp":1790709238956,"version":"4.1.0"},"reference-count":98,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Esthet Restor Dent"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To compare and critically evaluate dye\u2010based methods used for microleakage assessment, particularly focusing on dye penetration and dye extraction techniques, their advantages and drawbacks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>An electronic search of PubMed and Scopus databases was conducted to identify relevant articles from 2020 to 2025. After eligibility assessment, a total of 45 articles were included in this review, followed by data extraction and qualitative analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both dye penetration and dye extraction methods face significant challenges, including lack of standardization, methodological variability, and limited correlation with clinical outcomes. The identified confounding factors, such as anatomical variations, dye properties, sectioning errors, and measurement inconsistencies, heavily influence the reliability of results. While dye penetration enables localization of leakage pathways, it is limited by two\u2010dimensional analysis and random cutting axis. Dye extraction, although more objective, is destructive and lacks spatial information. Recommendations for protocol standardization and methodology improvement include the use of anatomically matched samples or 3D\u2010printed replicas, thermomechanical aging, fluorescent dyes and advanced imaging techniques for nondestructive three\u2010dimensional analysis, such as confocal laser scanning microscopy (CLSM) and micro\u2010computed tomography (micro\u2010CT).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Although in\u00a0vitro microleakage testing continues to face validity concerns, well\u2010designed studies with rigorous controls still provide valuable insights. Future research should focus on combining in\u00a0vitro techniques with in\u00a0vivo and long\u2010term clinical studies to improve the translation of findings into clinical practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jerd.70111","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T09:57:16Z","timestamp":1769767036000},"page":"1174-1187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Review of Dye Methods for Microleakage Assessment in Restorative Dentistry and Endodontics"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4142-9498","authenticated-orcid":false,"given":"Milan","family":"Mijkovic","sequence":"first","affiliation":[{"name":"Research Centre for Biomedicine, Faculty of Medicine University of Nis  Nis Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Dacic","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry and Endodontics, Faculty of Medicine University of Nis  Nis Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Popovic","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry and Endodontics, Faculty of Medicine University of Nis  Nis Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Nikolic","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry and Endodontics, Faculty of Medicine University of Nis  Nis Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nenad","family":"Stosic","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry and Endodontics, Faculty of Medicine University of Nis  Nis Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"issue":"2","key":"e_1_2_12_2_1","first-page":"262","article-title":"Coronal Microleakage and Its Impact on the Success of Endodontic Therapy: A Narrative Review","volume":"15","author":"Boshnjaku S.","year":"2025","journal-title":"International Journal of Biomedicine"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/jcd.jcd_1_23"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10015-1215"},{"issue":"6","key":"e_1_2_12_5_1","first-page":"99","article-title":"Microleakage in Endodontics","volume":"6","author":"Muliyar S.","year":"2014","journal-title":"Journal of International Oral Health"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13897"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-1546.2003.00041.x"},{"issue":"1","key":"e_1_2_12_8_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.14744\/eej.2018.13008","article-title":"Comparing the Ability of Different Materials and Techniques in Filling Artificial Internal Resorption Cavities","volume":"4","author":"Yazdi K. 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