{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T10:55:12Z","timestamp":1792407312246,"version":"4.4.0"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Little is known of <jats:italic>Toxoplasma gondii<\/jats:italic> isolates circulating in wildlife. The mouflon (<jats:italic>Ovis ammon<\/jats:italic>) is very popular game animal, hunted for its trophy horns. Here, we report the isolation and genetic characterization of <jats:italic>T. gondii<\/jats:italic> from two mouflons from Hawaii, <jats:styled-content style=\"fixed-case\">USA<\/jats:styled-content>. Both sheep had antibodies titers of 1:800 or higher. Viable <jats:italic>T. gondii<\/jats:italic> were isolated and nested <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> genotyping revealed two genotypes, a clonal Type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> (designated TgMouflon<jats:styled-content style=\"fixed-case\">US<\/jats:styled-content>1), and a new genotype (designated TgMouflon<jats:styled-content style=\"fixed-case\">US<\/jats:styled-content>2, and Toxo<jats:styled-content style=\"fixed-case\">DB PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> genotypes #249). This is the first report of <jats:italic>T. gondii<\/jats:italic> infection, isolation and genetic characterization in mouflons from the <jats:styled-content style=\"fixed-case\">USA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jeu.12151","type":"journal-article","created":{"date-parts":[[2014,7,26]],"date-time":"2014-07-26T14:02:55Z","timestamp":1406383375000},"page":"141-143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Toxoplasma gondii<\/i> Isolates from Mouflon Sheep (<i>Ovis ammon<\/i>) from Hawaii, <scp>USA<\/scp>"],"prefix":"10.1111","volume":"62","author":[{"given":"Shiv K.","family":"Verma","sequence":"first","affiliation":[{"name":"United States Department of Agriculture Agricultural Research Service Beltsville Agricultural Research Center Animal Parasitic Diseases Laboratory Building 1001 Beltsville Maryland 20705\u20102350"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Tennessee Knoxville Tennessee 37996\u20100845"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitender P.","family":"Dubey","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture Agricultural Research Service Beltsville Agricultural Research Center Animal Parasitic Diseases Laboratory Building 1001 Beltsville Maryland 20705\u20102350"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.03.033"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE-1126R.1"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.2307\/3283823"},{"key":"e_1_2_5_5_1","volume-title":"Toxoplasmosis of Animals and Humans","author":"Dubey J. P.","year":"2010"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1987.tb01426.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2011.06.005"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.11.013"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-33.3.480"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182013001844"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009991065"},{"key":"e_1_2_5_12_1","volume-title":"Mammals in Hawaii","author":"Tomich P. Q.","year":"1986"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002852"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-36.2.205"}],"container-title":["Journal of Eukaryotic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjeu.12151","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjeu.12151","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jeu.12151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T14:50:59Z","timestamp":1696258259000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jeu.12151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1111\/jeu.12151"],"URL":"https:\/\/doi.org\/10.1111\/jeu.12151","archive":["Portico"],"relation":{},"ISSN":["1066-5234","1550-7408"],"issn-type":[{"value":"1066-5234","type":"print"},{"value":"1550-7408","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11]]},"assertion":[{"value":"2014-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-06-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}