{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:37:18Z","timestamp":1761489438019},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T00:00:00Z","timestamp":1410739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["R25 60825-06","AI31788"],"award-info":[{"award-number":["R25 60825-06","AI31788"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Microsporidium, <jats:italic>Anncaliia algerae<\/jats:italic>, an obligate intracellular parasite, has been identified as an opportunistic human pathogen, but treatment has not been evaluated for infections with this organism. Albendazole, an antitubulin polymerization drug used against parasitic worm infections, has been the medication of choice used to treat some microsporidial infections affecting humans, with varying results ranging from clearing infection (<jats:italic>Encephalitozoon<\/jats:italic>) to resistance (<jats:italic>Enterocytozoon<\/jats:italic>). This study illustrates the effect of albendazole treatment on <jats:italic>A. algerae<\/jats:italic> infection in Rabbit Kidney (<jats:styled-content style=\"fixed-case\">RK<\/jats:styled-content>13) cells and Human Fetal Lung (<jats:styled-content style=\"fixed-case\">HFL<\/jats:styled-content>\u20101) fibroblasts. Albendazole appears to have an attenuating effect on <jats:italic>A. algerae<\/jats:italic> infection and albendazole's <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub> in <jats:styled-content style=\"fixed-case\">RK<\/jats:styled-content>13 cells is 0.1\u00a0\u03bcg\/ml. Long\u2010term treatment inhibits up to 98% of spore production, but interrupting treatment reestablishes the infection without new exposure to the parasite as supported by microscopic observations. The parasite's beta\u2010tubulin gene was purified, cloned, and sequenced. Five of the six specific amino acids, associated with benzimidazole sensitivity, are conserved in <jats:italic>A. algerae<\/jats:italic>. These findings suggest that <jats:italic>A. algerae<\/jats:italic> is sensitive to albendazole; however, the organism is not completely cleared from cultures.<\/jats:p>","DOI":"10.1111\/jeu.12160","type":"journal-article","created":{"date-parts":[[2014,8,8]],"date-time":"2014-08-08T14:16:51Z","timestamp":1407507411000},"page":"60-68","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Analysis of the Beta\u2010Tubulin Gene and Morphological Changes of the Microsporidium <i>Anncaliia algerae<\/i> both Suggest Albendazole Sensitivity"],"prefix":"10.1111","volume":"62","author":[{"given":"Marianita","family":"Santiana","sequence":"first","affiliation":[{"name":"Rutgers University Newark New Jersey 07102"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cyrilla","family":"Pau","sequence":"additional","affiliation":[{"name":"Rutgers University Newark New Jersey 07102"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter M.","family":"Takvorian","sequence":"additional","affiliation":[{"name":"Rutgers University Newark New Jersey 07102"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Cali","sequence":"additional","affiliation":[{"name":"Rutgers University Newark New Jersey 07102"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2006.00140.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0310420"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.1.55-61.2002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118395264.app1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.46.10.898"},{"key":"e_1_2_6_7_1","volume-title":"Microsporidia: Opportunistic Pathogens in Patients with AIDS","author":"Bryan R. 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