{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:19:04Z","timestamp":1791141544392,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,10,28]],"date-time":"2014-10-28T00:00:00Z","timestamp":1414454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Emerging methods based on mass spectrometry (<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) can be used in the rapid identification of microorganisms. Thus far, these practical and rapidly evolving methods have mainly been applied to characterize prokaryotes. We applied matrix\u2010assisted laser\u2010desorption\u2010ionization\u2010time\u2010of\u2010flight mass spectrometry <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content> in the analysis of whole cells of 18 <jats:italic>N. fowleri<\/jats:italic> isolates belonging to three genotypes. Fourteen originated from the cerebrospinal fluid or brain tissue of primary amoebic meningoencephalitis patients and four originated from water samples of hot springs, rivers, lakes or municipal water supplies. Whole <jats:italic>Naegleria<\/jats:italic> trophozoites grown in axenic cultures were washed and mixed with <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content> matrix. Mass spectra were acquired with a 4700 <jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> instrument. <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content> yielded consistent patterns for all isolates examined. Using a combination of novel data processing methods for visual peak comparison, statistical analysis and proteomics database searching we were able to detect several biomarkers that can differentiate all species and isolates studied, along with common biomarkers for all <jats:italic>N. fowleri<\/jats:italic> isolates. <jats:italic>Naegleria fowleri<\/jats:italic> could be easily separated from other species within the genus <jats:italic>Naegleria<\/jats:italic>. A number of peaks detected were tentatively identified. <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content> fingerprinting is a rapid, reproducible, high\u2010throughput alternative method for identifying <jats:italic>Naegleria<\/jats:italic> isolates. This method has potential for studying eukaryotic agents.<\/jats:p>","DOI":"10.1111\/jeu.12178","type":"journal-article","created":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T04:37:24Z","timestamp":1411015044000},"page":"12-20","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Detection of Biomarkers of Pathogenic <i>Naegleria fowleri<\/i> Through Mass Spectrometry and Proteomics"],"prefix":"10.1111","volume":"62","author":[{"given":"Hercules","family":"Moura","sequence":"first","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health Centers for Disease Control and Prevention M.S. 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