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It has been found in a wide range of animals and is considered an important zoonotic pathogen. To date, little information is available on the role that cattle play in the epidemiology of human microsporidiosis caused by <jats:italic>E. bieneusi<\/jats:italic> in China. In this study, 133 fecal specimens from dairy cattle were collected in Heilongjiang Province, China. <jats:italic>Enterocytozoon bieneusi<\/jats:italic> was identified and genotyped by nested <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis of the internal transcribed spacer (<jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content>) region of the <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene, with 30.1% positive. Nine <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> genotypes were identified: six known genotypes\u2014O (<jats:italic>n\u00a0<\/jats:italic>=\u00a026), EbpA (<jats:italic>n<\/jats:italic>\u00a0=\u00a02), I (<jats:italic>n<\/jats:italic>\u00a0=\u00a02), J (<jats:italic>n<\/jats:italic>\u00a0=\u00a02), D (<jats:italic>n<\/jats:italic>\u00a0=\u00a01) and <jats:styled-content style=\"fixed-case\">BEB<\/jats:styled-content>4 (<jats:italic>n<\/jats:italic>\u00a0=\u00a01)\u2014and three novel genotypes named as <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>\u2010I to <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> (two each). Genotype O was identified in cattle for the first time. The observation of all the six known genotypes here reported previously in humans, and also the fact of all the three novel genotypes (<jats:styled-content style=\"fixed-case\">CHN<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>1 to <jats:styled-content style=\"fixed-case\">CHN<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>3) falling into zoonotic group 1, indicate the possibility of cattle in the transmission of <jats:italic>E. bieneusi<\/jats:italic> to humans.<\/jats:p>","DOI":"10.1111\/jeu.12210","type":"journal-article","created":{"date-parts":[[2015,2,25]],"date-time":"2015-02-25T07:24:46Z","timestamp":1424849086000},"page":"553-560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["<i>Enterocytozoon bieneusi<\/i> in Dairy Cattle in the Northeast of China: Genetic Diversity of <scp>ITS<\/scp> Gene and Evaluation of 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