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In this fungus, the cell wall is exceptionally not the outermost structure due to the presence of a surrounding polysaccharide capsule, which has been highly studied. Considering that there is little information about <jats:italic>C. neoformans<\/jats:italic> cell wall composition, we aimed at describing proteins and lipids extractable from this organelle, using as model the acapsular mutant <jats:italic>C. neoformans<\/jats:italic> cap 67. Purified cell wall preparations were extracted with either chloroform\/methanol or hot sodium dodecyl sulfate. Total lipids fractionated in silica gel 60 were analyzed by electrospray ionization tandem mass spectrometry (<jats:styled-content style=\"fixed-case\">ESI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>), while trypsin digested proteins were analyzed by liquid chromatography coupled to tandem mass spectrometry (<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>). We detected 25 phospholipid species among phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, and phosphatidic acid. Two glycolipid species were identified as monohexosyl ceramides. We identified 192 noncovalently linked proteins belonging to different metabolic processes. Most proteins were classified as secretory, mainly via nonclassical mechanisms, suggesting a role for extracellular vesicles (EV) in transwall transportation. In concert with that, orthologs from 86% of these proteins have previously been reported both in fungal cell wall and\/or in EV. The possible role of the presently described structures in fungal\u2013host relationship is discussed.<\/jats:p>","DOI":"10.1111\/jeu.12213","type":"journal-article","created":{"date-parts":[[2015,3,2]],"date-time":"2015-03-02T19:55:10Z","timestamp":1425326110000},"page":"591-604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of Lipids and Proteins Associated to the Cell Wall of the Acapsular Mutant <i>Cryptococcus neoformans<\/i> Cap 67"],"prefix":"10.1111","volume":"62","author":[{"given":"Larissa V. G.","family":"Longo","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo EPM\u2010UNIFESP S\u00e3o Paulo 04023\u2010062 S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto S.","family":"Nakayasu","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Border Biomedical Research Center University of Texas at El Paso (UTEP) El Paso 79968\u20100519 Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jhon H. S.","family":"Pires","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo EPM\u2010UNIFESP S\u00e3o Paulo 04023\u2010062 S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Gazos\u2010Lopes","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Border Biomedical Research Center University of Texas at El Paso (UTEP) El Paso 79968\u20100519 Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milene C.","family":"Vallejo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo EPM\u2010UNIFESP S\u00e3o Paulo 04023\u2010062 S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago J. 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