{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:43:16Z","timestamp":1782135796774,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T00:00:00Z","timestamp":1435104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31330079"],"award-info":[{"award-number":["31330079"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Giardia duodenalis<\/jats:italic> is an important zoonotic pathogen, causes diarrhea in humans and animals worldwide. To date, few data are available on the prevalence of <jats:italic>G. duodenalis<\/jats:italic> in rabbits in China. In total, 955 fecal samples were collected from rabbits during 2008\u20132011 in Henan Province, Central China. The overall prevalence of <jats:italic>G. duodenalis<\/jats:italic> was 8.4% (80\/955) on microscopic analysis, with the highest infection rate (11.3%) in rabbits aged 91\u2013200\u00a0d. All <jats:italic>G. duodenalis<\/jats:italic>\u2010positive isolates were characterized at the small subunit ribosomal <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, \u03b2\u2010giardin (<jats:italic>bg<\/jats:italic>), glutamate dehydrogenase (<jats:italic>gdh<\/jats:italic>), and triosephosphate isomerase genes. Two assemblages and a mixed assemblage were detected in the rabbits: assemblage B (<jats:italic>n<\/jats:italic>\u00a0=\u00a026), assemblage E (<jats:italic>n<\/jats:italic>\u00a0=\u00a02), and a mixed assemblage of B and E (<jats:italic>n<\/jats:italic>\u00a0=\u00a04). Assemblage B isolates showed variability at the nucleotide sequences belonging to the so\u2010called subtype <jats:styled-content style=\"fixed-case\">BIV<\/jats:styled-content>, based on analysis of multiple genes. This is the first report of <jats:italic>G. duodenalis<\/jats:italic> assemblage E in rabbits, and one novel subtype of assemblage E was identified through sequence analysis of <jats:italic>gdh<\/jats:italic> and <jats:italic>bg<\/jats:italic> genes, respectively. Our data suggest that rabbits may be reservoirs of <jats:italic>G. duodenalis<\/jats:italic> cysts potentially infectious to humans.<\/jats:p>","DOI":"10.1111\/jeu.12239","type":"journal-article","created":{"date-parts":[[2015,6,3]],"date-time":"2015-06-03T16:40:38Z","timestamp":1433349638000},"page":"810-814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Prevalence of Zoonotic <i>Giardia duodenalis<\/i> Assemblage B and First Identification of Assemblage E in Rabbit Fecal Samples Isolates from Central China"],"prefix":"10.1111","volume":"62","author":[{"given":"Meng","family":"Qi","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450002 China"},{"name":"International Joint Research Laboratory for Zoonotic Diseases of Henan Zhengzhou 450002 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