{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T08:52:01Z","timestamp":1699951921569},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,6]],"date-time":"2016-05-06T00:00:00Z","timestamp":1462492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"UNAM-FESI","award":["FESI-DIP-PAPCA-2014-13"],"award-info":[{"award-number":["FESI-DIP-PAPCA-2014-13"]}]},{"name":"Spanish Ministry of Economy and Competitivity","award":["RYC-2011-08863","RD12\/0018\/0012","FIS PI13\/00490"],"award-info":[{"award-number":["RYC-2011-08863","RD12\/0018\/0012","FIS PI13\/00490"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The virulence of various amoebic parasites has been correlated with the presence of electron\u2010dense granules (<jats:styled-content style=\"fixed-case\">EDG<\/jats:styled-content>s) in the cytoplasm of trophozoites. Here, we report the finding by transmission electron microscopy of a large number of <jats:styled-content style=\"fixed-case\">EDG<\/jats:styled-content>s in a recent culture of <jats:italic>Acanthamoeba culbertsoni,<\/jats:italic> isolated from a severe case of human keratitis. When this isolate was maintained in culture for 6\u00a0mo, the granules almost disappeared. However, after induction of mice brain lesions with the long\u2010term cultured isolate, recovered amoebas had abundant <jats:styled-content style=\"fixed-case\">EDG<\/jats:styled-content>s. Trophozoites of the original isolate, or those recovered from experimental lesions, secreted <jats:styled-content style=\"fixed-case\">EDG<\/jats:styled-content>s into the medium when incubated with <jats:styled-content style=\"fixed-case\">MDCK<\/jats:styled-content> cells. To analyze a possible cytotoxic effect the conditioned medium was incubated with <jats:styled-content style=\"fixed-case\">MDCK<\/jats:styled-content> monolayers. After 5\u00a0h, the media containing <jats:styled-content style=\"fixed-case\">EDG<\/jats:styled-content>s produced opening of the tight junctions; at 24\u00a0h, cell viability was compromised, and at 48\u00a0h most of the cells were detached from the monolayer. In contrast, trophozoites in long\u2010term cultures did not release <jats:styled-content style=\"fixed-case\">EDG<\/jats:styled-content>s to the medium during incubation with <jats:styled-content style=\"fixed-case\">MDCK<\/jats:styled-content> cells, and the corresponding conditioned medium did not have any effect on <jats:styled-content style=\"fixed-case\">MDCK<\/jats:styled-content> monolayers. Our observations further support the hypothesis that <jats:styled-content style=\"fixed-case\">EDG<\/jats:styled-content>s play a role in the cytopathogenic mechanisms of <jats:italic>A.\u00a0culbertsoni<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jeu.12321","type":"journal-article","created":{"date-parts":[[2016,4,18]],"date-time":"2016-04-18T05:52:40Z","timestamp":1460958760000},"page":"744-750","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Acanthamoeba culbertsoni<\/i>: Electron\u2010Dense Granules in a Highly Virulent Clinical Isolate"],"prefix":"10.1111","volume":"63","author":[{"given":"Bibiana","family":"Ch\u00e1vez\u2010Mungu\u00eda","sequence":"first","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis Center for Research and Advanced Studies Mexico City 07360 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizbeth","family":"Salazar\u2010Villatoro","sequence":"additional","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis Center for Research and Advanced Studies Mexico City 07360 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maritza","family":"Oma\u00f1a\u2010Molina","sequence":"additional","affiliation":[{"name":"School of Superior Studies Iztacala National Autonomous University of Mexico Mexico City 54090 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha","family":"Espinosa\u2010Cantellano","sequence":"additional","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis Center for Research and Advanced Studies Mexico City 07360 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Ram\u00edrez\u2010Flores","sequence":"additional","affiliation":[{"name":"School of Superior Studies Iztacala National Autonomous University of Mexico Mexico City 54090 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Lorenzo\u2010Morales","sequence":"additional","affiliation":[{"name":"University Institute of Tropical Diseases and Public Health of the Canary Islands University of La Laguna Canary Islands 38203 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolfo","family":"Mart\u00ednez\u2010Palomo","sequence":"additional","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis Center for Research and Advanced Studies Mexico City 07360 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2004.09.001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2012.2376"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2007.01.003"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.1689-1693.2005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.25.15838"},{"key":"e_1_2_6_7_1","first-page":"41","article-title":"Disseminated Acanthamoeba sinusitis in patients with AIDS: a possible role for early antiretroviral therapy","volume":"14","author":"Carter W. W.","year":"2004","journal-title":"AIDS Read"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.21042"},{"key":"e_1_2_6_9_1","first-page":"235","article-title":"Ultrastructural study of Entamoeba invadens encystation and excystation","volume":"35","author":"Ch\u00e1vez\u2010Mungu\u00eda B.","year":"2003","journal-title":"J. Submicrosc. Cytol. Pathol."},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2008.00372.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2005.04-3273.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0447-x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2014.03.027"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/152165400300001534"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2006.02.004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.127.3313.1506"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1976.11687144"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2014.06.020"},{"key":"e_1_2_6_19_1","first-page":"197","article-title":"Proteolytic enzymes of pathogenic and non\u2010pathogenic strains of Acanthamoeba spp","volume":"44","author":"Hadas E.","year":"1993","journal-title":"Trop. Med. Parasitol."},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2006.44.1.15"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00047-10"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"2858","DOI":"10.1128\/JCM.38.8.2858-2861.2000","article-title":"Proteases as markers for differentiation of pathogenic and nonpathogenic species of Acanthamoeba","volume":"38","author":"Khan N. A.","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2006.44.4.321"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2000)086[0012:PACOAS]2.0.CO;2"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.7.2697-2700.1998"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)02833-8"},{"key":"e_1_2_6_27_1","first-page":"188","article-title":"Electron dense granules and the pathogenicity in Entamoeba histolytica","volume":"28","author":"Le\u00f3n G.","year":"1997","journal-title":"Arch. Med. Res."},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2015010"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45970-0"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2000.tb00007.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.1997.tb01076.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez\u2010Palomo A. Meza I. Ch\u00e1vez B. Rosales\u2010Encina J. L. Mu\u00f1oz M. L. Gonz\u00e1lez\u2010Robles A.&Rojkind M.1987.Entamoeba histolytica: Activation and release of membrane dense bodies. Host\u2010parasite cellular and molecular interactions in protozoal infections. NATO ASI Series H11.Springer Berlin Germany. pp.371\u2013376.","DOI":"10.1007\/978-3-642-72840-2_43"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1116"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2014.05.012"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb01919.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.02.004"},{"key":"e_1_2_6_37_1","first-page":"122","volume-title":"Culture collection of algae and protozoa","author":"Page F. C.","year":"1988"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.08.004"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2009.00399.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00356.x"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2007.00280.x"},{"key":"e_1_2_6_42_1","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1128\/CDLI.8.4.837-840.2001","article-title":"Cytopathic changes in rat microglial cells induced by pathogenic Acanthamoeba culbertsoni: morphology and cytokine relase","volume":"8","author":"Shin H. J.","year":"2001","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u20106\u201042"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1998.tb05068.x"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-015-1027-8"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(00)90110-5"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(92)90555-Q"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot087395"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4408-4413.2000"}],"container-title":["Journal of Eukaryotic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjeu.12321","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjeu.12321","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jeu.12321","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T01:02:43Z","timestamp":1696208563000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jeu.12321"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,6]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/jeu.12321"],"URL":"https:\/\/doi.org\/10.1111\/jeu.12321","archive":["Portico"],"relation":{},"ISSN":["1066-5234","1550-7408"],"issn-type":[{"value":"1066-5234","type":"print"},{"value":"1550-7408","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,6]]},"assertion":[{"value":"2015-08-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-05-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}