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However, resistance toward it is emerging and adverse effects occur in some patients. Studies in rodent models suggested that echinocandins could be useful to treat <jats:italic>Pneumocystis<\/jats:italic> pneumonia. Echinocandins inhibit the catalytic subunit Gsc1 of the enzymatic complex ensuring the synthesis of 1,3\u2010\u03b2 glucan, an essential constituent of cell walls of most fungi. Besides, inhibitors of the enzyme Kre6 involved in the synthesis of 1,6\u2010\u03b2 glucan, another essential component of fungal walls, were recently described. We identified and functionally characterized these two potential drug targets in the human pathogen <jats:italic>P.\u00a0jirovecii<\/jats:italic> by rescue of the null allele of the orthologous gene in <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. The <jats:italic>P.\u00a0jirovecii<\/jats:italic> proteins Gsc1 and Kre6 identified using those of the relative <jats:italic>Pneumocystis carinii<\/jats:italic> as the query sequence showed high sequence identity to the putative fungal orthologs (53\u201397% in conserved functional domains). The expression of their encoding genes on plasmid rescued the increased sensitivity to, respectively, caspofungin or calcofluor white of the corresponding <jats:italic>S.\u00a0cerevisiae<\/jats:italic> null allele. The uniqueness and likely essentiality of these proteins suggest that they are potential good drug targets.<\/jats:p>","DOI":"10.1111\/jeu.12385","type":"journal-article","created":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T11:22:43Z","timestamp":1479381763000},"page":"481-490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification and Functional Ascertainment of the <i>Pneumocystis jirovecii<\/i> Potential Drug Targets Gsc1 and Kre6 Involved in Glucan Synthesis"],"prefix":"10.1111","volume":"64","author":[{"given":"Amanda","family":"Luraschi","sequence":"first","affiliation":[{"name":"Institute of Microbiology Lausanne University Hospital and University of Lausanne Lausanne 1011 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ousmane H.","family":"Ciss\u00e9","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Lausanne University Hospital and 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