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This organism, first reported in Martinique Island, now has become an emerging infectious agent in Thailand. Symptomatic cases of<jats:italic>L<\/jats:italic>.\u00a0<jats:italic>martiniquensis<\/jats:italic>infection among humans have continuously increased. In the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and control disease transmission. The unsuccessful serological test using the commercial<jats:styled-content style=\"fixed-case\">rK<\/jats:styled-content>39 dipstick based on antigen from<jats:italic>Leishmania donovani<\/jats:italic>to detect the antibodies against<jats:styled-content style=\"fixed-case\">VL<\/jats:styled-content>among infected Thai patients has encouraged us to further explore a new sensitive and specific antigenic epitope. In this study, we determined the sequences and expressed recombinant proteins of kinesin 39 (<jats:italic>k<\/jats:italic>39), heat shock protein 70 (<jats:italic>hsp<\/jats:italic>70), heat shock protein 83 (<jats:italic>hsp<\/jats:italic>83), and glycoprotein 63 (<jats:italic>gp<\/jats:italic>63) of<jats:italic>L<\/jats:italic>.\u00a0<jats:italic>martiniquensis<\/jats:italic>to evaluate the diagnostic efficiency to detect antibodies against<jats:italic>L.\u00a0martiniquensis<\/jats:italic>in patient sera. The preliminary results from western blot analysis have suggested that K39 is the most sensitive recombinant protein to detect<jats:italic>L<\/jats:italic>.\u00a0<jats:italic>martiniquensis<\/jats:italic>. Moreover, this recombinant protein reacts with antibodies against<jats:italic>L.\u00a0donovani<\/jats:italic>and<jats:italic>Leishmania infantum<\/jats:italic>, making it a promising antigen for further development of a universal rapid diagnostic tool for<jats:styled-content style=\"fixed-case\">VL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jeu.12415","type":"journal-article","created":{"date-parts":[[2017,3,28]],"date-time":"2017-03-28T08:28:53Z","timestamp":1490689733000},"page":"820-828","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparison of Recombinant Proteins of Kinesin 39, Heat Shock Protein 70, Heat Shock Protein 83, and Glycoprotein 63 for Antibody Detection of<i>Leishmania martiniquensis<\/i>Infection"],"prefix":"10.1111","volume":"64","author":[{"given":"Suradej","family":"Siripattanapipong","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Science Mahidol University Bangkok 10400 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotomo","family":"Kato","sequence":"additional","affiliation":[{"name":"Division of Medical Zoology, Department of Infection and Immunity Jichi Medical University Tochigi 329\u20100498 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peerapan","family":"Tan\u2010ariya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Mahidol University Bangkok 10400 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathirut","family":"Mungthin","sequence":"additional","affiliation":[{"name":"Department of Parasitology Phramongkutklao College of Medicine Bangkok 10400 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2696-2075","authenticated-orcid":false,"given":"Saovanee","family":"Leelayoova","sequence":"additional","affiliation":[{"name":"Department of Parasitology Phramongkutklao College of Medicine Bangkok 10400 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03640.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.11-0707"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.2.775"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.13-0623"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001153"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90427-1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2014011"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05487.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"3979","DOI":"10.1128\/JCM.38.11.3979-3983.2000","article-title":"Improved immunodiagnosis of cystic hydatid disease by using a synthetic peptide with higher diagnostic value than that of its parent protein, Echinococcus granulosus antigen B","volume":"38","author":"Gonzalez\u2010Sapienza G.","year":"2000","journal-title":"J. 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