{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T11:45:48Z","timestamp":1771501548525,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,8,28]],"date-time":"2017-08-28T00:00:00Z","timestamp":1503878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572020"],"award-info":[{"award-number":["81572020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["26460516"],"award-info":[{"award-number":["26460516"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Acanthamoeba<\/jats:italic> spp. can be parasitic in certain\u00a0situations and are responsible for serious human infections, including <jats:italic>Acanthamoeba<\/jats:italic> keratitis, granulomatous amoebic encephalitis, and cutaneous acanthamoebiasis. We analyzed the fatty acid composition of <jats:italic>Acanthamoeba castellanii<\/jats:italic> trophozoites and tested the inhibitory activity of the main fatty acids, oleic acid and arachidonic acid, in\u00a0vitro. Oleic acid markedly inhibited the growth of <jats:italic>A.\u00a0castellanii<\/jats:italic>, with trophozoite viability of 57.4% at a concentration of 200\u00a0\u03bcM. Caspase\u20103 staining and annexin V assays showed that apoptotic death occurred in <jats:italic>A.\u00a0castellanii<\/jats:italic> trophozoites. Quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and dot blot analysis showed increased levels of metacaspase and interleukin\u20101\u03b2 converting enzyme, which is also an indication of apoptosis. In contrast, arachidonic acid showed negligible inhibition of growth of <jats:italic>A.\u00a0castellanii<\/jats:italic> trophozoites. Stimulated expression of <jats:italic>Atg3<\/jats:italic>,<jats:italic> Atg8<\/jats:italic> and <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3A\/B genes and monodansylcadaverine labeling suggested that oleic acid induces apoptosis by triggering autophagy of trophozoites.<\/jats:p>","DOI":"10.1111\/jeu.12454","type":"journal-article","created":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T16:26:44Z","timestamp":1502209604000},"page":"191-199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Apoptosis of <i>Acanthamoeba castellanii<\/i> Trophozoites Induced by Oleic Acid"],"prefix":"10.1111","volume":"65","author":[{"given":"Duo","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Fudan University School of Medicine Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Fudan University School of Medicine Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Fudan University School of Medicine Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongfeng","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Fudan University School of Medicine Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junlong","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Fudan University School of Medicine Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihong","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Fudan University School of Medicine Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Tachibana","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Tokai University School of Medicine Isehara Kanagawa 259\u20101193 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8851-6903","authenticated-orcid":false,"given":"Xunjia","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Fudan University School of Medicine Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-7489com"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00300-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04758-14"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-2355-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2009.05.004"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-115-1-233"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6257(98)00004-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.18.13315"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00067-9"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1042\/bst0180627","article-title":"Effect of growth temperature on fatty acid biosynthesis in Acanthamoeba castellanii","volume":"18","author":"Jones A. L.","year":"1990","journal-title":"Biochem. Soc. Trans."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01131"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00023.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.09.001"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/74994"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115678"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.13.3597"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M506928200"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)02294-9"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)78150-2"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21105"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.2.273-307.2003"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.111.184341"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1086\/518253"},{"key":"e_1_2_7_25_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.molbiopara.2009.06.005","article-title":"Autophagy protein 8 mediating autophagosome in encysting","volume":"168","author":"Moon E. K.","year":"2009","journal-title":"Acanthamoeba. Mol. Biochem. Parasitol."},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2011.49.2.103"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-011-0927-8"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101243"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(02)00272-4"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2929"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2013.06.003"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.2.254"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.10.014"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20020189"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605158200"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2004.01.002"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-006-9067-5"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-1-5"},{"key":"e_1_2_7_39_1","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1017\/S0031182007003459","article-title":"Primary structure, expression and localization of two intermediate subunit lectins of Entamoeba dispar that contain multiple CXXC motifs","volume":"134","author":"Tachibana H.","year":"2003","journal-title":"Parasitology"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.51.1.193"},{"key":"e_1_2_7_41_1","first-page":"229","article-title":"Apoptosis in procyclic Trypanosoma brucei rhodesiense in\u00a0vitro","volume":"3","author":"Welburn S. C.","year":"1996","journal-title":"Cell Death Differ."},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051619898"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208756200"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90485-9"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2004.08.003"}],"container-title":["Journal of Eukaryotic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjeu.12454","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jeu.12454","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T08:19:39Z","timestamp":1695629979000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jeu.12454"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,28]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1111\/jeu.12454"],"URL":"https:\/\/doi.org\/10.1111\/jeu.12454","archive":["Portico"],"relation":{},"ISSN":["1066-5234","1550-7408"],"issn-type":[{"value":"1066-5234","type":"print"},{"value":"1550-7408","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,28]]},"assertion":[{"value":"2017-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}