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Tyrosine\u2010rich proteins are well\u2010known components of the <jats:italic>Eimeria<\/jats:italic> oocyst wall. Recently, cysteine motif containing proteins (<jats:styled-content style=\"fixed-case\">COWP<\/jats:styled-content> family), as described in <jats:italic>Toxoplasma gondii<\/jats:italic> and <jats:italic>Cryptosporidium spp<\/jats:italic>., have also been characterized in <jats:italic>Eimeria<\/jats:italic>. Here, we identified a novel <jats:styled-content style=\"fixed-case\">COWP<\/jats:styled-content>\u2010related protein, En<jats:styled-content style=\"fixed-case\">OWP<\/jats:styled-content>13, and tracked it via transfection technology in <jats:italic>Eimeria nieschulzi<\/jats:italic>. The subsequent analysis suggests that the <jats:styled-content style=\"fixed-case\">mC<\/jats:styled-content>herry\u2010tagged En<jats:styled-content style=\"fixed-case\">OWP<\/jats:styled-content>13 localizes to the wall\u2010forming bodies I and the outer wall. Immunohistochemical analysis confirmed the distribution of wall\u2010forming bodies similar to avian <jats:italic>Eimeria<\/jats:italic> species and revealed that the wall\u2010forming bodies I show peroxidase activity. The En<jats:styled-content style=\"fixed-case\">OWP<\/jats:styled-content>13 amino acid composition and <jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>\u2010cadaverine\u2010positive wall\u2010forming bodies I suggest a participation of an enzyme with transglutaminase activity. This is the first description and characterization of this novel outer oocyst wall protein, which is also orthologous to other <jats:italic>Eimeria<\/jats:italic> species and <jats:italic>Toxoplasma gondii<\/jats:italic>, suggesting a new potential cross\u2010linking mechanism of wall\u2010forming proteins via isopeptide bonds.<\/jats:p>","DOI":"10.1111\/jeu.12498","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T12:22:23Z","timestamp":1515586943000},"page":"518-530","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Distribution and Processing of <i>Eimeria nieschulzi<\/i><scp>OWP<\/scp>13, a New Protein of the <scp>COWP<\/scp> Family"],"prefix":"10.1111","volume":"65","author":[{"given":"Stefanie","family":"Wiedmer","sequence":"first","affiliation":[{"name":"Faculty of Biology Institute of Zoology Technische Universit\u00e4t Dresden Zellescher Weg 20 B Dresden 01217 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Buder","sequence":"additional","affiliation":[{"name":"Faculty of Biology Institute of Zoology Technische Universit\u00e4t Dresden Zellescher Weg 20 B Dresden 01217 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinja","family":"Bleischwitz","sequence":"additional","affiliation":[{"name":"Faculty of Biology Institute of Zoology Technische Universit\u00e4t Dresden Zellescher Weg 20 B Dresden 01217 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4811-6171","authenticated-orcid":false,"given":"Michael","family":"Kurth","sequence":"additional","affiliation":[{"name":"Faculty of Biology Institute of Zoology Technische Universit\u00e4t Dresden Zellescher Weg 20 B Dresden 01217 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,31]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00345-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2006.07.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.3.456-464.2003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.10.1775"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/591520"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2004.03.011"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(03)00185-1"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012002247"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep29030"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm981"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2012.01.010"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0982-3"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-007-0868-1"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-1192-0"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2011.06.001"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762009000200022"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2011.07.001"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi963168z"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-001-0524-0"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-8274-2158-6"},{"key":"e_1_2_8_22_1","first-page":"32","article-title":"Effect of sodium hypochlorite on the oocyst wall of Eimeria tenella as shown by Electron Microscopy","volume":"37","author":"Nyberg P. 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