{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T21:51:54Z","timestamp":1766181114554,"version":"3.37.3"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702025"],"award-info":[{"award-number":["81702025"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> (<jats:italic>T.\u00a0gondii<\/jats:italic>) rhoptry proteins (Tg<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>s) have been considered main targets and indicator molecules for immune diagnosis and prophylaxis since they initially present during the process of invasion. In this study, the effect of intramuscularly injecting the genetic vaccine <jats:styled-content style=\"fixed-case\">pVAX<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>22 was evaluated, made by inserting the Tg<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>22 sequence into the eukaryotic expression vector of <jats:styled-content style=\"fixed-case\">pVAX<\/jats:styled-content> I, into <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice. The levels of IgG, IgG1, and IgG2a in <jats:styled-content style=\"fixed-case\">pVAX<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>22 vaccinated animals were integrally increased. It was uncovered by cytokine profile analyses that the levels of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 were significantly increased, while no significant changes were detected in <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 levels. In addition, we found that immunization with <jats:styled-content style=\"fixed-case\">pVAX<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>22 significantly prolonged the survival time (13.80\u00a0\u00b1\u00a01.75\u00a0d) of mice after challenge infection with the virulent <jats:italic>T.\u00a0gondii <\/jats:italic><jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> strain, in comparison with those of control animals (died within 10\u00a0d). Moreover, the number of brain cysts (1,406\u00a0\u00b1\u00a0277) in the animals subjected to <jats:styled-content style=\"fixed-case\">pVAX<\/jats:styled-content>\u2010Tg<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>22 vaccination decreased remarkably (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) compared with the blank control mice (2,333\u00a0\u00b1\u00a0473), and the size of brain cysts in <jats:styled-content style=\"fixed-case\">pVAX<\/jats:styled-content>\u2010Tg<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>22 group was significantly smaller than the groups of blank, <jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">pVAXI<\/jats:styled-content>. These results suggested that Tg<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>22 as <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine could trigger strong humoral and cellular responses and induce partial protection against toxoplasmosis.<\/jats:p>","DOI":"10.1111\/jeu.12639","type":"journal-article","created":{"date-parts":[[2018,6,2]],"date-time":"2018-06-02T09:46:19Z","timestamp":1527932779000},"page":"147-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The Molecular Characterization and Immunity Identification of Rhoptry Protein 22 of <i>Toxoplasma gondii<\/i> as a <scp>DNA<\/scp> Vaccine Candidate Against Toxoplasmosis"],"prefix":"10.1111","volume":"66","author":[{"given":"Zhenchao","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences Xinxiang Medical University Xinxiang Henan 453003 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