{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T03:44:43Z","timestamp":1784432683802,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T00:00:00Z","timestamp":1534204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["237990"],"award-info":[{"award-number":["237990"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Trichomonas vaginalis<\/jats:italic> is the etiological agent of human trichomoniasis. Metronidazole has high treatment success rate among trichomoniasis patients. However, metronidazole\u2010resistant <jats:italic>T.\u00a0vaginalis<\/jats:italic> has been reported, contributing in an increasing number of refractory cases. The mechanism of metronidazole resistance in this parasite is still unclear. In the vaginal environment, where the microaerophilic conditions prevail but the iron concentration is constantly fluctuating, the metronidazole resistance profile of <jats:italic>T.\u00a0vaginalis<\/jats:italic> could be altered. In this study, we developed metronidazole\u2010resistant strains of <jats:italic>T.\u00a0vaginalis<\/jats:italic> and evaluate if iron availability is important to the action of the drug. The modulation of iron levels and iron chelation affected the actions of metronidazole both in susceptible and resistant strains. Interestingly, the early resistant strains exhibited minor iron content. The results of transcription analysis in the early resistant strains showed dysregulation in the expression of genes that codified proteins involved in iron transporter, iron\u2013sulfur cluster assemblage, and oxidative stress response, which could not be observed in the late resistant and susceptible strains. Our results indicate that iron content plays an important role in the metronidazole action in <jats:italic>T.\u00a0vaginalis<\/jats:italic> and likely to be related to iron\u2013sulfur proteins involved in metronidazole activation and oxidative stress via Fenton reaction.<\/jats:p>","DOI":"10.1111\/jeu.12671","type":"journal-article","created":{"date-parts":[[2018,7,26]],"date-time":"2018-07-26T09:19:34Z","timestamp":1532596774000},"page":"309-315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Role of Iron Status in the Early Progression of Metronidazole Resistance in <i>Trichomonas vaginalis<\/i> Under Microaerophilic Conditions"],"prefix":"10.1111","volume":"66","author":[{"given":"Wendy","family":"Arg\u00e1ez\u2010Correa","sequence":"first","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica y Gen\u00e9tica Molecular Facultad de Qu\u00edmica de la Universidad Aut\u00f3noma de Yucat\u00e1n M\u00e9rida 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