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Many <jats:styled-content style=\"fixed-case\">CBP<\/jats:styled-content>s share a type of Ca<jats:sup>2+<\/jats:sup>\u2010binding domain known as the <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content>\u2010hand. The <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content>\u2010hand motif has been well studied and consists of a helix\u2010loop\u2010helix structural domain with specific amino acids in the loop region that interact with Ca<jats:sup>2+<\/jats:sup>. In <jats:italic>Toxoplasma gondii<\/jats:italic> a large number of genes (approximately 68) are predicted to have at least one <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content>\u2010hand motif. The majority of these genes have not been characterized. We report the characterization of two <jats:styled-content style=\"fixed-case\">EF<\/jats:styled-content>\u2010hand motif\u2010containing proteins, <jats:italic>Tg<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>1_216620<\/jats:italic> and <jats:italic>Tg<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>1_280480<\/jats:italic>, which localize to the plasma membrane and to the rhoptry bulb, respectively. Genetic disruption of these genes by <jats:styled-content style=\"fixed-case\">CRISPR<\/jats:styled-content> (clustered regularly interspaced short palindromic repeats)\/Cas9 (<jats:styled-content style=\"fixed-case\">CRISPR<\/jats:styled-content>\u2010associated protein 9) resulted in mutant parasite clones (\u0394<jats:italic>tg216620<\/jats:italic> and \u0394<jats:italic>tg280480<\/jats:italic>) that grew at a slower rate than control cells. Ca<jats:sup>2+<\/jats:sup> measurements showed that \u0394<jats:italic>tg216620<\/jats:italic> cells did not respond to extracellular Ca<jats:sup>2+<\/jats:sup> as the parental controls while \u0394<jats:italic>tg280480<\/jats:italic> cells appeared to respond as the parental cells. Our hypothesis is that <jats:italic>Tg<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>1_216620<\/jats:italic> is important for Ca<jats:sup>2+<\/jats:sup> influx while <jats:italic>Tg<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>1_280480<\/jats:italic> may be playing a different role in the rhoptries.<\/jats:p>","DOI":"10.1111\/jeu.12675","type":"journal-article","created":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T12:14:01Z","timestamp":1533125641000},"page":"343-353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of Two EF\u2010hand Domain\u2010containing Proteins from <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"66","author":[{"given":"Le","family":"Chang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Jilin University Changchun 130062 China"},{"name":"Center for Tropical and Emerging Global Diseases University of Georgia Athens Georgia 30602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Dykes","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases University of Georgia Athens Georgia 30602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Jilin University Changchun 130062 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2041-6295","authenticated-orcid":false,"given":"Silvia N.J.","family":"Moreno","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases University of Georgia Athens Georgia 30602"},{"name":"Department of Cellular Biology University of Georgia Athens Georgia 30602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miryam Andrea","family":"Hortua Triana","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases University of Georgia Athens Georgia 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