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Infection occurs when amoebae enter the nasal cavity, attach to the nasal mucosa and travel along olfactory neurons towards the olfactory bulb. Upon reaching the central nervous system, the amoebae replicate very rapidly and can cause death in 3\u201310\u00a0days. Little is known about the conditions in which the amoeba can survive in the environment. We have tested conditions beyond the known boundaries on the viability of amoebae by introducing them into moderate and extreme salinity, <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>, and temperatures. Our data shows that although viability expectedly decreases towards each of these extreme conditions, their tolerance was much greater than anticipated, including viability in moderate salinity, a wide <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> range, and temperatures higher than the previously reported 45\u00a0\u00b0C.<\/jats:p>","DOI":"10.1111\/jeu.12719","type":"journal-article","created":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T11:38:58Z","timestamp":1550057938000},"page":"752-756","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["The Effect of Different Environmental Conditions on the Viability of <i>Naegleria fowleri<\/i> Amoebae"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7208-7692","authenticated-orcid":false,"given":"Charlton","family":"Lam","sequence":"first","affiliation":[{"name":"Department of Microbiology &amp; Immunology Virginia Commonwealth University Richmond Virginia 23219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"He","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology Virginia Commonwealth University Richmond Virginia 23219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francine","family":"Marciano\u2010Cabral","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology Virginia Commonwealth University Richmond Virginia 23219"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1128\/am.28.1.64-65.1974","article-title":"Hemin replaces serum as a growth requirement for Naegleria","volume":"28","author":"Band R. 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