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The results showed that the overall infection rates of <jats:italic>Giardia duodenalis<\/jats:italic> and <jats:italic>Enterocytozoon bieneusi<\/jats:italic> were 0.8% (5\/620) and 15% (93\/620), respectively, in Tibetan sheep, and 0% (0\/260) and 9.6% (25\/260), respectively, in Tibetan goats. Based on sequence analysis of the SSU rRNA, <jats:italic>tpi<\/jats:italic>,<jats:italic> bg<\/jats:italic>, and <jats:italic>gdh<\/jats:italic> genes of <jats:italic>G.\u00a0duodenalis<\/jats:italic>, only assemblage E was identified. Based on sequence analysis of the ribosomal internal transcriptional spacer (ITS) region of <jats:italic>E. bieneusi<\/jats:italic>, a total of 12 genotypes (three novel and nine known) were detected, and these clustered into two separate phylogenetic groups. Genotypes CHG19, EbpA, EbpC, H, PigEBITS5, and CTS3 clustered into Group 1 with high zoonotic potential, while genotypes BEB6, CHC8, CHG1, I, CTS1, and CTS2 fell within the host\u2010specific Group 2. Ten genotypes were detected in Tibetan sheep, and two genotypes were found in Tibetan goats. The current study indicated that <jats:italic>E.\u00a0bieneusi<\/jats:italic> infections are widespread among these livestock, and Tibetan goats may play an important role as a reservoir of zoonotic <jats:italic>E.\u00a0bieneusi<\/jats:italic> genotypes.<\/jats:p>","DOI":"10.1111\/jeu.12758","type":"journal-article","created":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T20:14:05Z","timestamp":1566591245000},"page":"100-106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Molecular Characterization of <i>Giardia duodenalis<\/i> and <i>Enterocytozoon bieneusi<\/i> Isolated from Tibetan Sheep and Tibetan Goats Under Natural Grazing Conditions in Tibet"],"prefix":"10.1111","volume":"67","author":[{"given":"Yankai","family":"Chang","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yange","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yayun","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwen","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiakui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan Hubei 430070 China"},{"name":"Laboratory of Detection and Monitoring of Highland Animal Disease Tibet Agriculture and Animal Husbandry College Linzhi 860000 Tibet China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongjun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuchun","family":"Jian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changshen","family":"Ning","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-131X","authenticated-orcid":false,"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5081-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.447-475.2001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07744-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.5.2595-2599.2002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2005.06.013"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.08.022"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32834aa152"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw361"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02803-10"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X15000656"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00033-10"},{"key":"e_1_2_8_13_1","first-page":"193","article-title":"High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil","volume":"59","author":"Fm P. 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