{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:41:08Z","timestamp":1785739268837,"version":"3.56.0"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Allocation from University Grants Commission of Bangladesh"},{"name":"Doctoral Scientific Research Start-up Foundation from Henan University of Chinese Medicine","award":["00104311-2019-31"],"award-info":[{"award-number":["00104311-2019-31"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To determine the occurrence and genotypes of <jats:italic>Enterocytozoon bieneusi<\/jats:italic> in captive mammals at Bangladesh National Zoo and to assess their zoonotic significance, 200 fecal samples from 32 mammalian species were examined using a nested <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and sequencing of internal transcribed spacer (<jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content>) gene. <jats:italic>Enterocytozoon bieneusi<\/jats:italic> was detected in 16.5% (33\/200) of the samples. Seven different <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> genotypes were identified, including two known genotypes (D and J) and five new ones (<jats:styled-content style=\"fixed-case\">BAN<\/jats:styled-content>4 to <jats:styled-content style=\"fixed-case\">BAN<\/jats:styled-content>8). Genotype D was the most common genotype being observed in 19 isolates. In phylogenetic analysis, four genotypes (D, <jats:styled-content style=\"fixed-case\">BAN<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">BAN<\/jats:styled-content>5, and <jats:styled-content style=\"fixed-case\">BAN<\/jats:styled-content>6), detected in 30 isolates (90.9%), belonged to Group 1 having zoonotic potential. The sequence of genotype J found in a Malayan pangolin was clustered in so\u2010called ruminant\u2010specific Group 2. The other two genotypes <jats:styled-content style=\"fixed-case\">BAN<\/jats:styled-content>7 and <jats:styled-content style=\"fixed-case\">BAN<\/jats:styled-content>8 were clustered in primate\u2010specific Group 5. To our knowledge, this is the first report of molecular characterization of <jats:italic>E.\u00a0bieneusi<\/jats:italic> in Bangladesh, particularly in captive\u2010bred wildlife in this country. The potentially zoonotic genotypes of <jats:italic>E.\u00a0bieneusi<\/jats:italic> are maintained in zoo mammals that may transmit among these animals and to the humans through environmental contamination or contact.<\/jats:p>","DOI":"10.1111\/jeu.12792","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T02:03:45Z","timestamp":1583114625000},"page":"427-435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Evidence for Zoonotic Potential of <i>Enterocytozoon bieneusi<\/i> in Its First Molecular Characterization in Captive Mammals at Bangladesh National Zoo"],"prefix":"10.1111","volume":"67","author":[{"given":"Md Robiul","family":"Karim","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science Bangabandhu Sheikh Mujibur Rahman Agricultural University Gazipur 1706 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzana Islam","family":"Rume","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Public Health Patuakhali Science and Technology University Barishal 8210 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abu Nasar Md Aminoor","family":"Rahman","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science Bangabandhu Sheikh Mujibur Rahman Agricultural University Gazipur 1706 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6417-0901","authenticated-orcid":false,"given":"Junqiang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"},{"name":"Academy of Chinese Medical Sciences Henan University of Chinese Medicine Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-131X","authenticated-orcid":false,"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3427-6"},{"key":"e_1_2_9_3_1","first-page":"497","article-title":"The role of wildlife in emerging and re\u2010emerging zoonoses","volume":"23","author":"Bengis R. 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