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Previously, <jats:italic>E<\/jats:italic>.\u00a0<jats:italic>tenella<\/jats:italic> serine\/threonine protein phosphatase (<jats:italic>Et<\/jats:italic><jats:styled-content style=\"fixed-case\">STP<\/jats:styled-content>) was found to be differentially expressed in drug\u2010sensitive (<jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content>) and drug\u2010resistant strains using <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010seq. In the present study, we found that transcription and translation levels of <jats:italic>Et<\/jats:italic><jats:styled-content style=\"fixed-case\">STP<\/jats:styled-content> were higher in diclazuril\u2010resistant (<jats:styled-content style=\"fixed-case\">DZR<\/jats:styled-content>) strains and maduramicin\u2010resistant (<jats:styled-content style=\"fixed-case\">MRR<\/jats:styled-content>) strains than in <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content> strains using quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>) and Western blotting. Enzyme activity results indicated that the catalytic activity of <jats:italic>Et<\/jats:italic><jats:styled-content style=\"fixed-case\">STP<\/jats:styled-content> was higher in the two drug\u2010resistant strains than in <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content> strains. Western blot and <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> analysis also showed that expression levels of <jats:italic>Et<\/jats:italic><jats:styled-content style=\"fixed-case\">STP<\/jats:styled-content> were higher in unsporulated oocysts (<jats:styled-content style=\"fixed-case\">UO<\/jats:styled-content>) and second\u2010generation merozoites (<jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content>). Indirect immunofluorescence localization showed that <jats:italic>Et<\/jats:italic><jats:styled-content style=\"fixed-case\">STP<\/jats:styled-content> was located in most areas of the parasite with the exception of refractile bodies, and fluorescence intensity was enhanced during development. In vitro inhibition experiments showed that the ability of sporozoites (<jats:styled-content style=\"fixed-case\">SZ<\/jats:styled-content>) to invade cells was significantly decreased after treatment with anti\u2010<jats:styled-content style=\"fixed-case\">r<jats:italic>E<\/jats:italic><jats:italic>t<\/jats:italic>STP<\/jats:styled-content> antibody. These results indicated that <jats:italic>Et<\/jats:italic><jats:styled-content style=\"fixed-case\">STP<\/jats:styled-content> acted mainly during the developmental and reproductive stages of the parasite and may be related to the resistance of coccidia to external drug pressure.<\/jats:p>","DOI":"10.1111\/jeu.12798","type":"journal-article","created":{"date-parts":[[2020,5,2]],"date-time":"2020-05-02T03:45:08Z","timestamp":1588391108000},"page":"510-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular characterization of serine\/threonine protein phosphatase of <i>Eimeria tenella<\/i>"],"prefix":"10.1111","volume":"67","author":[{"given":"Yu","family":"Yu","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Parasitology of Ministry of Agriculture Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences Minhang Shanghai 200241 China"},{"name":"College of Life 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