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Chlamydia\u2010like bacteria have been described as symbionts of free\u2010living amoebae and other phylogenetically diverse eukaryotic hosts. In the present work, a systematic survey of the bacterial diversity associated with the ciliate <jats:italic>Euplotes octocarinatus<\/jats:italic> strain Zam5b\u20101 was performed, using metagenomic screening as well as classical full\u2010cycle rRNA approach, and a novel chlamydial symbiont was characterized. The metagenomic screening revealed 16S rRNA gene sequences from <jats:italic>Polynucleobacter necessarius<\/jats:italic>, three previously reported accessory symbionts, and a novel chlamydia\u2010like bacterium. Following the full\u2010cycle rRNA approach, we obtained the full\u2010length 16S rRNA gene sequence of this chlamydia\u2010like bacterium and developed probes for diagnostic fluorescence in situ hybridizations. The phylogenetic analysis of the 16S rRNA gene sequences unambiguously places the new bacterium in the family <jats:italic>Rhabdochlamydiaceae<\/jats:italic>. This is the first report of chlamydia\u2010like bacterium being found in <jats:italic>Euplotes<\/jats:italic>. Based on the obtained data, the bacterium is proposed as a new candidate genus and species: \u201c<jats:italic>Candidatus<\/jats:italic> Euplotechlamydia quinta.\u201d<\/jats:p>","DOI":"10.1111\/jeu.12945","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T10:43:47Z","timestamp":1661856227000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["\u201c<i>Candidatus<\/i> Euplotechlamydia quinta,\u201d a novel chlamydia\u2010like bacterium hosted by the ciliate <i>Euplotes octocarinatus<\/i> (Ciliophora, Spirotrichea)"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1164-6002","authenticated-orcid":false,"given":"Ruanlin","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology Shanxi University  Taiyuan 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