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Reptile hosts show potential to facilitate transmission of zoonotic trypanosomiases and offer key information to understanding the genus of <jats:italic>Trypanosoma<\/jats:italic>. Several species of squamates from different localities in South Africa were screened molecularly and microscopically for trypanosomes in the present study. Based on the combination of morphological and molecular analyses, two new species of <jats:italic>Trypanosoma<\/jats:italic>, <jats:italic>Trypanosoma<\/jats:italic> (<jats:italic>Squamatrypanum<\/jats:italic>) <jats:italic>ndumoensis<\/jats:italic> n. sp. and <jats:italic>Trypanosoma<\/jats:italic> (<jats:italic>Trypanosoma<\/jats:italic>) <jats:italic>tokoloshi<\/jats:italic> n. sp., infecting South African cordylid lizards (Cordylidae: Cordylinae) are described in this study. The first molecular data for a South African reptile trypanosome is provided herewith.<\/jats:p>","DOI":"10.1111\/jeu.12970","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T08:05:40Z","timestamp":1677225940000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Morphological and molecular diagnosis of two new species of <i>Trypanosoma<\/i> Gruby, 1843 infecting South African cordylid lizards (Squamata: Cordylidae: Cordylinae), <i>Trypanosoma<\/i> (<i>Squamatrypanum<\/i>) <i>ndumoensis<\/i> n. sp. and <i>Trypanosoma<\/i> (<i>Trypanosoma<\/i>) <i>tokoloshi<\/i> n. sp."],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6800-8007","authenticated-orcid":false,"given":"Bernard J.","family":"Jordaan","sequence":"first","affiliation":[{"name":"African Amphibian Conservation Research Group, Unit for Environmental Sciences and Management North\u2010West University  Potchefstroom South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7803-8768","authenticated-orcid":false,"given":"Johann","family":"van As","sequence":"additional","affiliation":[{"name":"Department of Zoology and Entomology University of the Free State  Phuthaditjhaba South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1856-0586","authenticated-orcid":false,"given":"Edward C.","family":"Netherlands","sequence":"additional","affiliation":[{"name":"African Amphibian Conservation Research Group, Unit for Environmental Sciences and Management North\u2010West University  Potchefstroom South Africa"},{"name":"Department of Zoology and Entomology University of the Free State  Bloemfontein South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0229536"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20107998.1973.tb05340.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2016.01.005"},{"key":"e_1_2_7_5_1","first-page":"609","article-title":"Sur quelques trypanosomes des vert\u00e9br\u00e9s asang froid de l\u2019Afrique Occidentale Fran\u00e7aise","volume":"66","author":"Bouet G.","year":"1909","journal-title":"Comptes Rendus des Seances de la Soci\u00e9t\u00e9 de Biologie et de ses Filiales"},{"issue":"2","key":"e_1_2_7_6_1","first-page":"133","article-title":"H\u00e9matozoaires de reptiles Malgaches. 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