{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:52:38Z","timestamp":1791420758434,"version":"4.3.3"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI19CIII\/00029"],"award-info":[{"award-number":["PI19CIII\/00029"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. is among the most frequent intestinal protists identified in humans globally. However, characterization of\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    subtype diversity in humans is ongoing. We report here the identification of novel\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    subtype ST41 in a Colombian patient undergoing colorectal cancer screening involving colonoscopy and fecal testing (microscopy, culture, PCR). The full\u2010length\n                    <jats:italic>ssu<\/jats:italic>\n                    rRNA gene sequence of the protist was generated using MinION long\u2010read sequencing technology. The validity of the novel subtype was confirmed via phylogenetic and pairwise distance analyses of the full\u2010length ST41 sequence and all other valid subtypes. The study provides reference material essential for conducting subsequent experimental studies.\n                  <\/jats:p>","DOI":"10.1111\/jeu.12978","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T10:58:13Z","timestamp":1684321093000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Identification and validation of novel\n                    <i>Blastocystis<\/i>\n                    subtype\n                    <scp>ST41<\/scp>\n                    in a Colombian patient undergoing colorectal cancer screening"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0731-6078","authenticated-orcid":false,"given":"Carolina","family":"Hern\u00e1ndez\u2010Castro","sequence":"first","affiliation":[{"name":"Parasitology Reference and Research Laboratory Spanish National Centre for Microbiology, Health Institute Carlos III  Madrid 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