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The presence of <jats:italic>Blastocystis<\/jats:italic> spp. was determined using polymerase chain reaction based on the small subunit rRNA (<jats:italic>SSU<\/jats:italic> rRNA) locus. The results revealed an overall infection rate of 26.3% (162\/617). Samples from Farm 1 in Luopu County showed the highest infection rate (76.3%, 29\/38). The highest and lowest infection rates were detected in the &lt;30\u2010day (34.4%, 43\/125) and\u2009&gt;\u200990\u2010day age groups (12.4%, 11\/89), respectively. The infection rate decreased with increasing age. Statistical analysis showed significant differences in the infection rates of <jats:italic>Blastocystis<\/jats:italic> spp. among the different sampling sites (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and age groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Four <jats:italic>Blastocystis<\/jats:italic> spp. subtypes (ST6, ST7, ST10, and ST23) were identified. The infection rates of the zoonotic subtypes, ST6 and ST7, were 3.2% (20\/617) and 22.2% (137\/617), respectively. The presence of <jats:italic>Blastocystis<\/jats:italic> spp. and zoonotic subtypes provided evidence for the potential transmission of this pathogen between Hotan Black chickens and humans, especially in animal handlers in this area.<\/jats:p>","DOI":"10.1111\/jeu.13012","type":"journal-article","created":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T03:34:32Z","timestamp":1700278472000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Molecular characterization of <i>Blastocystis<\/i> spp. in Hotan Black chickens in southern Xinjiang"],"prefix":"10.1111","volume":"71","author":[{"given":"Xinwei","family":"Feng","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Tarim University  Xinjiang China"},{"name":"Tarim Animal Disease Diagnosis and Control Engineering Laboratory of Xinjiang Production and Construction Corps  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyao","family":"Xin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Tarim University  Xinjiang China"},{"name":"Tarim Animal Disease Diagnosis and Control Engineering Laboratory of Xinjiang Production and Construction Corps  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Tarim University  Xinjiang China"},{"name":"Tarim Animal Disease Diagnosis and Control Engineering Laboratory of Xinjiang Production and Construction Corps  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Tarim University  Xinjiang China"},{"name":"Tarim Animal Disease Diagnosis and Control Engineering Laboratory of Xinjiang Production and Construction Corps  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Tarim University  Xinjiang China"},{"name":"Tarim Animal Disease Diagnosis and Control Engineering Laboratory of Xinjiang Production and Construction Corps  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0031-1549","authenticated-orcid":false,"given":"Fuchang","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Tarim University  Xinjiang China"},{"name":"Tarim Animal Disease Diagnosis and Control Engineering Laboratory of Xinjiang Production and Construction Corps  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4662-0398","authenticated-orcid":false,"given":"Meng","family":"Qi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Tarim University  Xinjiang China"},{"name":"Tarim Animal Disease Diagnosis and Control Engineering Laboratory of Xinjiang Production and Construction Corps  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,17]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"304","article-title":"Molecular characterization of Blastocystis isolates infecting farm animals in Victoria and Pila, Laguna","volume":"99","author":"Adao D.E.V.","year":"2016","journal-title":"Philippine Agricultural Scientist"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.12.011"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002002640"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vprsr.2017.11.012"},{"key":"e_1_2_6_6_1","first-page":"553","article-title":"The ultrastructure of Blastocystis galli from chickens","volume":"32","author":"Belova L.M.","year":"1998","journal-title":"Parazitologiia"},{"key":"e_1_2_6_7_1","first-page":"164","article-title":"Blastocystis galli sp. n. 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