{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:32:41Z","timestamp":1792542761709,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T00:00:00Z","timestamp":1705276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003841","name":"Ilam University of Medical Sciences","doi-asserted-by":"publisher","award":["A\u201010\u20102429\u201020"],"award-info":[{"award-number":["A\u201010\u20102429\u201020"]}],"id":[{"id":"10.13039\/501100003841","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A total of 360 fecal samples were randomly collected from 150 cattle, 150 sheep, and 60 humans (30 people with close animal contact and 30 individuals without close animal contact) at 10 farms in Ilam, western Iran from June 2022 to August 2023. All samples were directly examined for <jats:italic>Blastocystis<\/jats:italic> by zinc sulfate flotation, followed by microscopic observation. Positive samples were further subtyped using conventional PCR and sequencing methods. A mean prevalence of 5.3% (16\/300) was estimated for <jats:italic>Blastocystis<\/jats:italic> infection among examined animals, with 6% and 4.7% for cattle and sheep, respectively. Among the people who had close and non\u2010close animal contact, 16.7% (5\/30) and 3.3% (1\/30) were infected with <jats:italic>Blastocystis<\/jats:italic>, respectively (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). All 22 positive samples were successfully sequenced at the SSU rRNA locus. Accordingly, <jats:italic>Blastocystis<\/jats:italic> isolates infecting domestic animals in Ilam belonged to the four STs (ST1\u2010ST3, and ST10). Of the 16 animal isolates, nine sequences (four ST10, three ST3, and two ST1) were related to cattle, and seven sequences (three ST10, two ST3, and two ST2) were isolated from sheep. Among the six human isolates, ST3 was the most predominant ST, followed by STs 1, 2, 6, and 7 (one case each). Of note, ST1\u2010ST3 were isolated in various farms both from animals and their breeders, which indicates the possible circulation of these STs between animal and human populations.<\/jats:p>","DOI":"10.1111\/jeu.13019","type":"journal-article","created":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T06:29:05Z","timestamp":1705386545000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["First molecular characterization of <i>Blastocystis<\/i> subtypes from domestic animals (sheep and cattle) and their animal\u2010keepers in Ilam, western Iran: A zoonotic concern"],"prefix":"10.1111","volume":"71","author":[{"given":"Morteza","family":"Shams","sequence":"first","affiliation":[{"name":"Zoonotic Diseases Research Center Ilam University of Medical Sciences  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Mohammad","family":"Bahrami","sequence":"additional","affiliation":[{"name":"Faculty of Para\u2010Veterinary Medicine Ilam University  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Mousivand","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Veterinary Medicine Shiraz University  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laya","family":"Shamsi","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Faculty of Veterinary Medicine Urmia University  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4005-2687","authenticated-orcid":false,"given":"Ali","family":"Asghari","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, School of Medicine Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed","family":"Shahabi","sequence":"additional","affiliation":[{"name":"Department of Biology and Control of Disease Vectors, School of Health Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Sadrebazzaz","sequence":"additional","affiliation":[{"name":"Razi Vaccine &amp; Serum Research Institute Agricultural Research, Education and Extension Organization  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11686-021-00360-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-021-16187-1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2018.11.013"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2021.105183"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11686-020-00186-2"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10091693"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-389"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2021.101695"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407706-5.00001-0"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parepi.2020.e00134"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2975-5"},{"key":"e_1_2_10_13_1","first-page":"296","article-title":"Detection of Blastocystis subtypes in children with functional abdominal pain and celiac disease in \u00c7orum, Turkey","volume":"17","author":"G\u00fcreser A.S.","year":"2022","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.732129"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3641-2"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.819887"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2665-0"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-130"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2022.101848"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parepi.2020.e00138"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11010046"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11686-020-00322-y"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2019.104053"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.12.032"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2021.105085"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050677"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2244-9"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11686-022-00589-3"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2021.101650"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-022-05351-2"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42399-020-00285-1"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02541-12"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2016.05.015"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.17"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2019.12.009"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169637"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-3106-z"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103962"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.11.029"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-020-06761-w"}],"container-title":["Journal of Eukaryotic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jeu.13019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T13:32:33Z","timestamp":1715779953000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jeu.13019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,15]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1111\/jeu.13019"],"URL":"https:\/\/doi.org\/10.1111\/jeu.13019","archive":["Portico"],"relation":{},"ISSN":["1066-5234","1550-7408"],"issn-type":[{"value":"1066-5234","type":"print"},{"value":"1550-7408","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,15]]},"assertion":[{"value":"2023-10-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13019"}}