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As with other vertebrate models, underlying diseases and infections often impact research. Beyond mortality and morbidity, these conditions can compromise research end points by producing nonprotocol induced variation within experiments. <jats:italic>Pseudoloma neurophilia<\/jats:italic>, a microsporidium that targets the central nervous system, is the most frequently diagnosed pathogen in zebrafish facilities. The parasite undergoes direct, horizontal transmission within populations, and is also maternally transmitted with spores in ovarian fluid and occasionally within eggs. This transmission explains the wide distribution among research laboratories as new lines are generally introduced as embryos. The infection is chronic, and fish apparently never recover following the initial infection. However, most fish do not exhibit outward clinical signs. Histologically, the parasite occurs as aggregates of spores throughout the midbrain and spinal cord and extends to nerve roots. It often elicits meninxitis, myositis, and myodegeneration when it infects the muscle. There are currently no described therapies for the parasite, thus the infection is best avoided by screening with PCR\u2010based tests and removal of infected fish from a facility. Examples of research impacts include reduced fecundity, behavioral changes, transcriptome alterations, and autofluorescent lesions.<\/jats:p>","DOI":"10.1111\/jeu.13040","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T06:44:21Z","timestamp":1720075461000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Review of <i>Pseudoloma neurophilia<\/i> (Microsporidia): A common neural parasite of laboratory zebrafish (<i>Danio rerio<\/i>)"],"prefix":"10.1111","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9147-9146","authenticated-orcid":false,"given":"Corbin J.","family":"Schuster","sequence":"first","affiliation":[{"name":"Department of Natural Science Heritage University  Toppenish Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina N.","family":"Murray","sequence":"additional","affiliation":[{"name":"Zebrafish International 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